Antibody Name:	Anti-FKBP5 Antibody
Antibody SKU:	CAB3863
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 308-457 of human FKBP5 (NP_001139247.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HT-1080, SH-SY5Y, Jurkat, Mouse liver, Mouse kidney, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 308-457 of human FKBP5 (NP_001139247.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ESKA SESF LLAA FLNL AMCY LKLR EYTK AVEC CDKA LGLD SANE KGLY RRGE AQLL MNEF ESAK GDFE KVLE VNPQ NKAA RLQI SMCQ KKAK EHNE RDRR IYAN MFKK FAEQ DAKE EANK AMGK KTSE GVTN EKGT DSQA MEEE KPEG HV
Gene ID:	2289
Uniprot:	Q13451
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	30kDa/51kDa
Observed MW:	51kDa

Synonyms:

FKBP5, AIG6, FKBP51, FKBP54, P54, PPIase, Ptg-10

Background:

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It is thought to mediate calcineurin inhibition. It also interacts functionally with mature hetero-oligomeric progesterone receptor complexes along with the 90 kDa heat shock protein and P23 protein. This gene has been found to have multiple polyadenylation sites. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	FKBP5: Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Part of a heteromultimeric cytoplasmic complex with HSP90, HSP70 and steroid receptors. Dissociates from the complex when NR3C1 binds glucocorticoid. By androgen. Widely expressed, enriched in testis compared to other tissues. Inhibited by FK506 but not cyclosporin.
UniProt Protein Details:	Protein type:EC 5.2.1.8; Isomerase; Chaperone Chromosomal Location of Human Ortholog: 6p21.31 Cellular Component: nucleoplasm; endoplasmic reticulum membrane; membrane Molecular Function:protein binding; peptidyl-prolyl cis-trans isomerase activity; FK506 binding; heat shock protein binding Biological Process: protein peptidyl-prolyl isomerization; protein folding Disease: Major Depressive Disorder
NCBI Summary:	The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It is thought to mediate calcineurin inhibition. It also interacts functionally with mature hetero-oligomeric progesterone receptor complexes along with the 90 kDa heat shock protein and P23 protein. This gene has been found to have multiple polyadenylation sites. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
UniProt Code:	Q13451
NCBI GenInfo Identifier:	2851536
NCBI Gene ID:	2289
NCBI Accession:	Q13451.2
UniProt Related Accession:	Q13451
Molecular Weight:	51kDa
NCBI Full Name:	Peptidyl-prolyl cis-trans isomerase FKBP5
NCBI Synonym Full Names:	FKBP prolyl isomerase 5
NCBI Official Symbol:	FKBP5
NCBI Official Synonym Symbols:	P54; AIG6; FKBP51; FKBP54; PPIase; Ptg-10
NCBI Protein Information:	peptidyl-prolyl cis-trans isomerase FKBP5
UniProt Protein Name:	Peptidyl-prolyl cis-trans isomerase FKBP5
UniProt Synonym Protein Names:	51 kDa FK506-binding protein; 51 kDa FKBP; FKBP-51; 54 kDa progesterone receptor-associated immunophilin; Androgen-regulated protein 6; FF1 antigen; FK506-binding protein 5; FKBP-5; FKBP54; p54; HSP90-binding immunophilin; Rotamase
Protein Family:	FK506-binding protein
UniProt Gene Name:	FKBP5
UniProt Entry Name:	FKBP5_HUMAN

	Western blot analysis of extracts of various cell lines, using FKBP5 antibody (CAB3863) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human colon using FKBP5 antibody (CAB3863) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using FKBP5 antibody (CAB3863) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human uterus using FKBP5 antibody (CAB3863) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using FKBP5 antibody (CAB3863) at dilution of 1:100. Blue: DAPI for nuclear staining.