Antibody Name:	Anti-FKBP4 Antibody
Antibody SKU:	CAB5643
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 220-459 of human FKBP4 (NP_002005.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	293T, HeLa, Jurkat, MCF7, BT-474, SW620, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 220-459 of human FKBP4 (NP_002005.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YLKP SYAF GSVG KEKF QIPP NAEL KYEL HLKS FEKA KESW EMNS EEKL EQST IVKE RGTV YFKE GKYK QALL QYKK IVSW LEYE SSFS NEEA QKAQ ALRL ASHL NLAM CHLK LQAF SAAI ESCN KALE LDSN NEKG LFRR GEAH LAVN DFEL ARAD FQKV LQLY PNNK AAKT QLAV CQQR IRRQ LARE KKLY ANMF ERLA EEEN KAKA EASS GDHP TDTE MKEE QKSN TAGS QSQV ETEA
Gene ID:	2288
Uniprot:	Q02790
Cellular Location:	Cytoplasm, Mitochondrion, Nucleus, cytoskeleton, cytosol
Calculated MW:	51kDa
Observed MW:	58kDa

Synonyms:

FKBP4, FKBP51, FKBP52, FKBP59, HBI, Hsp56, PPIase, p52

Background:

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene.

UniProt Protein Function:	FKBP4: Immunophilin protein with PPIase and co-chaperone activities. Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
UniProt Protein Details:	Protein type:EC 5.2.1.8; Chaperone; Isomerase Chromosomal Location of Human Ortholog: 12p13.33 Cellular Component: cytoplasm; cytosol; endoplasmic reticulum membrane; nucleoplasm Molecular Function:FK506 binding; heat shock protein binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; protein binding, bridging Biological Process: negative regulation of microtubule polymerization or depolymerization; protein folding
NCBI Summary:	The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq, Sep 2008]
UniProt Code:	Q02790
NCBI GenInfo Identifier:	399866
NCBI Gene ID:	2288
NCBI Accession:	Q02790.3
UniProt Secondary Accession:	Q02790,Q9UCP1, Q9UCV7, D3DUQ1,
UniProt Related Accession:	Q02790
Molecular Weight:	51,805 Da
NCBI Full Name:	Peptidyl-prolyl cis-trans isomerase FKBP4
NCBI Synonym Full Names:	FK506 binding protein 4
NCBI Official Symbol:	FKBP4
NCBI Official Synonym Symbols:	HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
NCBI Protein Information:	peptidyl-prolyl cis-trans isomerase FKBP4
UniProt Protein Name:	Peptidyl-prolyl cis-trans isomerase FKBP4
UniProt Synonym Protein Names:	51 kDa FK506-binding protein; FKBP51
Protein Family:	Peptidyl-prolyl cis-trans isomerase
UniProt Gene Name:	FKBP4
UniProt Entry Name:	FKBP4_HUMAN

	Western blot analysis of extracts of various cell lines, using FKBP4 antibody (CAB5643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human lung cancer using FKBP4 Rabbit pAb (CAB5643) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using FKBP4 antibody (CAB5643). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug FKBP4 antibody (CAB5643). Western blot was performed from the immunoprecipitate using FKBP4 antibody (CAB5643) at a dilition of 1:1000.