Epigenetics & Nuclear Signaling Antibodies 5

Anti-Fibrillarin Antibody (CAB0850)

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SKU:
CAB0850
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-Fibrillarin Antibody
Antibody SKU:CAB0850
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human Fibrillarin
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HepG2, Jurkat, Mouse spleen, Rat liver
Immunogen:A synthesized peptide derived from human Fibrillarin
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:2091
Uniprot:P22087
Cellular Location:
Calculated MW:33kDa
Observed MW:37KDa
Anti-Fibrillarin AntibodyWestern blot - Fibrillarin Rabbit mAb (CAB0850)
Synonyms:FIB, FLRN, RNU3IP1
Background:This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]
UniProt Protein Function:FBL: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with NOLC1. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5. Belongs to the methyltransferase superfamily. Fibrillarin family.
UniProt Protein Details:

Protein type:EC 2.1.1.-; RNA-binding; Nucleolus; Methyltransferase; RNA processing

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Cajal body; granular component; membrane; nucleolus; nucleus

Molecular Function:protein binding; RNA binding

Biological Process: osteoblast differentiation; snoRNA metabolic process; tRNA processing; rRNA processing

NCBI Summary:This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]
UniProt Code:P22087
NCBI GenInfo Identifier:14549159
NCBI Gene ID:2091
NCBI Accession:P22087.2
UniProt Secondary Accession:P22087,O75259, Q6IAT5, Q9UPI6, B5BUE8,
UniProt Related Accession:P22087
Molecular Weight:321
NCBI Full Name:rRNA 2'-O-methyltransferase fibrillarin
NCBI Synonym Full Names:fibrillarin
NCBI Official Symbol:FBL  
NCBI Official Synonym Symbols:FIB; FLRN; RNU3IP1  
NCBI Protein Information:rRNA 2'-O-methyltransferase fibrillarin; 34-kD nucleolar scleroderma antigen; histone-glutamine methyltransferase; 34 kDa nucleolar scleroderma antigen; RNA, U3 small nucleolar interacting protein 1
UniProt Protein Name:rRNA 2'-O-methyltransferase fibrillarin
UniProt Synonym Protein Names:34 kDa nucleolar scleroderma antigen; Histone-glutamine methyltransferase
Protein Family:rRNA 2'-O-methyltransferase fibrillarin
UniProt Gene Name:FBL  
UniProt Entry Name:FBRL_HUMAN
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