Cell Biology Antibodies 6

Anti-FHOD1 Antibody (CAB15438)

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SKU:
CAB15438
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-FHOD1 Antibody
Antibody SKU:CAB15438
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1010-1120 of human FHOD1 (NP_037373.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1010-1120 of human FHOD1 (NP_037373.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TRGR MITE TEKF SGVA GEAP SNPS VPVA VSSG PGRG DADS HASM KSLL TSRP EDTT HNRR SRGM VQSS SPIM PTVG PSTA SPEE PPGS SLPS DTSD EIMD LLVQ SVTK SSP
Gene ID:29109
Uniprot:Q9Y613
Cellular Location:Cell projection, Cytoplasm, bleb, cytoskeleton
Calculated MW:126kDa
Observed MW:145kDa
Synonyms:FHOD1, FHOS
Background:This gene encodes a protein which is a member of the formin/diaphanous family of proteins. The gene is ubiquitously expressed but is found in abundance in the spleen. The encoded protein has sequence homology to diaphanous and formin proteins within the Formin Homology (FH)1 and FH2 domains. It also contains a coiled-coil domain, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. It is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:FHOD1: a cytoskeletal protein required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. Self-associates via the FH2 domain. Binds to F-actin via its N-terminus. Binds to the cytoplasmic domain of CD21 via its C- terminus.
UniProt Protein Details:

Protein type:Actin-binding

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: cytoplasm; membrane; nucleus; stress fiber

Molecular Function:identical protein binding; protein binding

Biological Process: positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes a protein which is a member of the formin/diaphanous family of proteins. The gene is ubiquitously expressed but is found in abundance in the spleen. The encoded protein has sequence homology to diaphanous and formin proteins within the Formin Homology (FH)1 and FH2 domains. It also contains a coiled-coil domain, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. It is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
UniProt Code:Q9Y613
NCBI GenInfo Identifier:62512187
NCBI Gene ID:29109
NCBI Accession:Q9Y613.3
UniProt Secondary Accession:Q9Y613,Q59F76, Q6Y1F2, Q76MS8, Q8N521,
UniProt Related Accession:Q9Y613
Molecular Weight:126,551 Da
NCBI Full Name:FH1/FH2 domain-containing protein 1
NCBI Synonym Full Names:formin homology 2 domain containing 1
NCBI Official Symbol:FHOD1  
NCBI Official Synonym Symbols:FHOS  
NCBI Protein Information:FH1/FH2 domain-containing protein 1
UniProt Protein Name:FH1/FH2 domain-containing protein 1
UniProt Synonym Protein Names:Formin homolog overexpressed in spleen 1; FHOS; Formin homology 2 domain-containing protein 1
Protein Family:FH1/FH2 domain-containing protein
UniProt Gene Name:FHOD1  
UniProt Entry Name:FHOD1_HUMAN
Anti-FHOD1 Antibody (CAB15438)Western blot analysis of extracts of HeLa cells, using FHOD1 antibody (CAB15438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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