Antibody Name:	Anti-FGF2[KO Validated] Antibody
Antibody SKU:	CAB11488
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human FGF2

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SKOV3

Immunogen:	A synthesized peptide derived from human FGF2
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	2247
Uniprot:	P09038
Cellular Location:	Nucleus, Secreted
Calculated MW:	31kDa
Observed MW:	18KDa/22KDa/31KDa

	Western blot analysis of extracts from normal (control) and FGF2 knockout (KO) HeLa cells, using FGF2 Rabbit mAb (CAB11488) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts of SKOV3 cells, using FGF2 Rabbit mAb (CAB11488) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human colon using [KO Validated] FGF2 Rabbit mAb (CAB11488) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] FGF2 Rabbit mAb (CAB11488) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] FGF2 Rabbit mAb (CAB11488) at dilution of 1:100 (40x lens).

Synonyms:

BFGF, FGF-2, FGFB, HBGF-2

Background:

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]

UniProt Protein Function:	FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:	Protein type:Activator; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 4q26 Cellular Component: extracellular space; extracellular region; nucleus Molecular Function:heparin binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; growth factor activity; cytokine activity; chemoattractant activity; fibroblast growth factor receptor binding Biological Process: extracellular matrix organization and biogenesis; wound healing; nerve growth factor receptor signaling pathway; somatic stem cell maintenance; activation of MAPK activity; positive regulation of transcription, DNA-dependent; chemotaxis; signal transduction; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; positive regulation of MAP kinase activity; positive chemotaxis; positive regulation of cell proliferation; embryonic morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; regulation of angiogenesis; positive regulation of angiogenesis; organ morphogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; release of sequestered calcium ion into cytosol; positive regulation of cell division; Ras protein signal transduction; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; inositol phosphate biosynthetic process
NCBI Summary:	The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
UniProt Code:	P09038
NCBI GenInfo Identifier:	261260095
NCBI Gene ID:	2247
NCBI Accession:	P09038.3
UniProt Secondary Accession:	P09038,O00527, P78443, Q16443, Q5PY50, Q7KZ11, Q7KZ72 Q9UC54, Q9UCS5, Q9UCS6, A4LBB8,
UniProt Related Accession:	P09038
Molecular Weight:
NCBI Full Name:	Fibroblast growth factor 2
NCBI Synonym Full Names:	fibroblast growth factor 2 (basic)
NCBI Official Symbol:	FGF2
NCBI Official Synonym Symbols:	BFGF; FGFB; FGF-2; HBGF-2
NCBI Protein Information:	fibroblast growth factor 2; prostatropin; heparin-binding growth factor 2; basic fibroblast growth factor bFGF
UniProt Protein Name:	Fibroblast growth factor 2
UniProt Synonym Protein Names:	Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
Protein Family:	Fibroblast growth factor
UniProt Gene Name:	FGF2
UniProt Entry Name:	FGF2_HUMAN

Antibodies	ELISA Kits
Anti-FGF2 Antibody (CAB0235)	Human FGF2 / bFGF ELISA Kit
FGF2 Antibody (PACO02215)	Human bFGF/FGF2 (Basic Fibroblast Growth Factor) CLIA Kit (HUES00347)
	Human Heparin-binding growth factor 2 (FGF2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)