Antibody Name:	Anti-FGF12 Antibody
Antibody SKU:	CAB2667
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human FGF12 (NP_004104.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human FGF12 (NP_004104.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MESK EPQL KGIV TRLF SQQG YFLQ MHPD GTID GTKD ENSD YTLF NLIP VGLR VVAI QGVK ASLY VAMN GEGY LYSS DVFT PECK FKES VFEN YYVI YSST LYRQ QESG RAWF LGLN KEGQ IMKG NRVK KTKP SSHF VPKP IEVC MYRE PSLH EIGE KQGR SRKS SGTP TMNG GKVV NQDS T
Gene ID:	2257
Uniprot:	P61328
Cellular Location:	Nucleus
Calculated MW:	20kDa/27kDa
Observed MW:	20kDa

Synonyms:

FGF12, EIEE47, FGF12B, FHF1

Background:

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.

UniProt Protein Function:	FGF12: Probably involved in nervous system development and function. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell development/differentiation; Cytokine Chromosomal Location of Human Ortholog: 3q28 Cellular Component: extracellular space; intracellular; nucleus Molecular Function:fibroblast growth factor receptor binding; growth factor activity; heparin binding; protein binding; sodium channel regulator activity Biological Process: adult locomotory behavior; cell-cell signaling; fibroblast growth factor receptor signaling pathway; heart development; JNK cascade; nervous system development; neuromuscular process; signal transduction; synaptic transmission
NCBI Summary:	The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	P61328
NCBI GenInfo Identifier:	47117683
NCBI Gene ID:	2257
NCBI Accession:	P61328.1
UniProt Secondary Accession:	P61328,O35339, P70376, Q8TBG5, Q92912, Q93001, B2R6B7 B2R976,
UniProt Related Accession:	P61328
Molecular Weight:	20,424 Da
NCBI Full Name:	Fibroblast growth factor 12
NCBI Synonym Full Names:	fibroblast growth factor 12
NCBI Official Symbol:	FGF12
NCBI Official Synonym Symbols:	FHF1; FGF12B
NCBI Protein Information:	fibroblast growth factor 12
UniProt Protein Name:	Fibroblast growth factor 12
UniProt Synonym Protein Names:	Fibroblast growth factor homologous factor 1; FHF-1; Myocyte-activating factor
Protein Family:	Fibroblast growth factor
UniProt Gene Name:	FGF12
UniProt Entry Name:	FGF12_HUMAN

	Western blot analysis of extracts of various cell lines, using FGF12 antibody (CAB2667) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat testis using FGF12 antibody (CAB2667) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using FGF12 antibody (CAB2667) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using FGF12 antibody (CAB2667) at dilution of 1:150 (40x lens).