Name: Anti-FEZ1 Antibody (CAB15361)
Brand: Signal Transduction Antibodies 2
SKU: CAB15361
Availability: OutOfStock

Antibody Name:	Anti-FEZ1 Antibody
Antibody SKU:	CAB15361
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human FEZ1 (NP_005094.1).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human
Positive Samples:	U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human FEZ1 (NP_005094.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEAP LVSL DEEF EDLR PSCS EDPE EKPQ CFYG SSPH HLED PSLS ELEN FSSE IISF KSME DLVN EFDE KLNV CFRN YNAK TENL APVK NQLQ IQEE EETL QDEE VWDA LTDN YIPS LSED WRDP NIEA LNGN CSDT EIHE KEEE EFNE KSEN DSGI NEEP
Gene ID:	9638
Uniprot:	Q99689
Cellular Location:	Cell membrane, Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	12kDa/45kDa
Observed MW:	71kDa

Synonyms:

FEZ1, UNC-76

Background:

This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.

UniProt Protein Function:	FEZ1: May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. Able to restore partial locomotion and axonal fasciculation to C.elegans unc-76 mutants in germline transformation experiments. May participate in the transport of mitochondria and other cargos along microtubules. Belongs to the zygin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 11q24.2 Cellular Component: microtubule cytoskeleton; centrosome; microtubule; mitochondrion; axon; cytoplasm; dendrite; plasma membrane Molecular Function:protein binding; gamma-tubulin binding; protein kinase C binding; protein N-terminus binding Biological Process: establishment of mitochondrion localization; axon guidance; nervous system development; transport; cell adhesion
NCBI Summary:	This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99689
NCBI GenInfo Identifier:	13431526
NCBI Gene ID:	9638
NCBI Accession:	Q99689.1
UniProt Secondary Accession:	Q99689,O00679, O00728, Q6IBI7,
UniProt Related Accession:	Q99689
Molecular Weight:	12,074 Da
NCBI Full Name:	Fasciculation and elongation protein zeta-1
NCBI Synonym Full Names:	fasciculation and elongation protein zeta 1 (zygin I)
NCBI Official Symbol:	FEZ1
NCBI Protein Information:	fasciculation and elongation protein zeta-1; zygin I; zygin-1
UniProt Protein Name:	Fasciculation and elongation protein zeta-1
UniProt Synonym Protein Names:	Zygin I; Zygin-1
Protein Family:	Fasciculation and elongation protein
UniProt Gene Name:	FEZ1
UniProt Entry Name:	FEZ1_HUMAN

Western blot analysis of extracts of U-251MG cells, using FEZ1 antibody (CAB15361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

Anti-FEZ1 Antibody (CAB15362)

FEZ1 Antibody (PACO09279)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)