Signal Transduction Antibodies 2
Anti-FEM1A Antibody (CAB16129)
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- SKU:
- CAB16129
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-FEM1A Antibody |
| Antibody SKU: | CAB16129 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human FEM1A (NP_061178.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 293T, K-562, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human FEM1A (NP_061178.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RSLL RRGA SVNR TTRT NSTP LRAA CFDG HLEV VRYL VGEH QADL EVAN RHGH TCLM ISCY KGHR EIAR YLLE QGAQ VNRR SAKG NTAL HDCA ESGS LEIL QLLL GCKA RMER DGYG MTPL LAAS VTGH TNIV EYLI QEQP GQEQ VAGG EAQP GLPQ EDPS TSQG CAQP QGAP CCSS SPEE PLNG ESYE SCCP TSRE AAVE A |
| Gene ID: | 55527 |
| Uniprot: | Q9BSK4 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 73kDa |
| Observed MW: | 80kDa |
| Synonyms: | FEM1A, EPRAP |
| Background: |
| UniProt Protein Function: | FEM1A: Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. May participate in antiinflammatory signaling via its interaction with PTGER4. Belongs to the fem-1 family. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Ligase Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: cytoplasm Molecular Function:EP4 subtype prostaglandin E2 receptor binding; ubiquitin-protein ligase activity Biological Process: regulation of ubiquitin-protein ligase activity; negative regulation of inflammatory response; protein ubiquitination |
| UniProt Code: | Q9BSK4 |
| NCBI GenInfo Identifier: | 24308163 |
| NCBI Gene ID: | 55527 |
| NCBI Accession: | NP_061178.1 |
| UniProt Secondary Accession: | Q9BSK4,Q711P8, Q9NPN7, Q9NPW8, B2RDI3, |
| UniProt Related Accession: | Q9BSK4 |
| Molecular Weight: | 73,639 Da |
| NCBI Full Name: | protein fem-1 homolog A |
| NCBI Synonym Full Names: | fem-1 homolog a (C. elegans) |
| NCBI Official Symbol: | FEM1A |
| NCBI Official Synonym Symbols: | EPRAP |
| NCBI Protein Information: | protein fem-1 homolog A |
| UniProt Protein Name: | Protein fem-1 homolog A |
| UniProt Synonym Protein Names: | FEM1-alpha; Prostaglandin E receptor 4-associated protein |
| Protein Family: | Protein |
| UniProt Gene Name: | FEM1A |
| UniProt Entry Name: | FEM1A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FEM1A antibody (CAB16129) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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