Metabolism Antibodies 3
Anti-FECH Antibody (CAB8418)
- SKU:
- CAB8418
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-FECH Antibody |
| Antibody SKU: | CAB8418 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human FECH (NP_000131.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, HT-29, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human FECH (NP_000131.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MRSL GANM AAAL RAAG VLLR DPLA SSSW RVCQ PWRW KSGA AAAA VTTE TAQH AQGA KPQV QPQK RKPK TGIL MLNM GGPE TLGD VHDF LLRL FLDR DLMT LPIQ NKLA PFIA KRRT PKIQ EQYR RIGG GSPI KIWT SKQG EGMV KLLD ELSP NTAP HKYY IGFR YVHP LTEE AIEE MERD GLER AIAF TQYP QYSC STTG |
| Gene ID: | 2235 |
| Uniprot: | P22830 |
| Cellular Location: | Matrix side, Mitochondrion inner membrane, Peripheral membrane protein |
| Calculated MW: | 47kDa/48kDa |
| Observed MW: | 50kDa |
| Synonyms: | FECH, EPP, FCE |
| Background: | The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3. |
| UniProt Code: | P22830 |
| NCBI GenInfo Identifier: | 60499021 |
| NCBI Gene ID: | 2235 |
| NCBI Accession: | NP_000131 |
| UniProt Secondary Accession: | P22830,Q8IXN1, Q8NAN0, A8KA72, |
| UniProt Related Accession: | P22830 |
| Molecular Weight: | 48,625 Da |
| NCBI Full Name: | ferrochelatase, mitochondrial isoform b |
| NCBI Synonym Full Names: | ferrochelatase |
| NCBI Official Symbol: | FECH |
| NCBI Official Synonym Symbols: | EPP; FCE |
| NCBI Protein Information: | ferrochelatase, mitochondrial; heme synthase; heme synthetase; protoheme ferro-lyase; protoporphyria |
| UniProt Protein Name: | Ferrochelatase, mitochondrial |
| UniProt Synonym Protein Names: | Heme synthase; Protoheme ferro-lyase |
| UniProt Gene Name: | FECH |
| UniProt Entry Name: | HEMH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FECH antibody (CAB8418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded human stomach using FECH antibody (CAB8418) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using FECH antibody (CAB8418) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using FECH antibody (CAB8418) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse stomach using FECH antibody (CAB8418) at dilution of 1:100 (40x lens). |
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