Anti-FDX1/ADX Antibody (CAB20895)
- SKU:
- CAB20895
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Plant
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-FDX1/ADX Antibody (CAB20895) |
| Antibody SKU: | CAB20895 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunofluorescence |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of Human FDX1/ADX. |
| Application: | Western blotting, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, A-549, U-87MG, Mouse liver, Mouse testis, Mouse kidney, Rat testis, Rat kidney |
| Immunogen: | Recombinant protein of Human FDX1/ADX. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 19kDa |
| Observed MW: | 13KDa |
| Synonyms: | FDX1, ADX, FDX, LOH11CR1D |
| Background: | This gene encodes a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to mitochondrial cytochrome P450, involved in steroid, vitamin D, and bile acid metabolism. Pseudogenes of this functional gene are found on chromosomes 20 and 21. |
 | Western blot analysis of extracts of various cell lines, using FDX1/ADX antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
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