Cell Biology Antibodies 9

Anti-FBXW7 Antibody (CAB5872)

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SKU:
CAB5872
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-FBXW7 Antibody
Antibody SKU:CAB5872
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 408-707 of human FBXW7 (NP_361014.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, SH-SY5Y, MCF7, mouse testis, rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 408-707 of human FBXW7 (NP_361014.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AVTG KCLR TLVG HTGG VWSS QMRD NIII SGST DRTL KVWN AETG ECIH TLYG HTST VRCM HLHE KRVV SGSR DATL RVWD IETG QCLH VLMG HVAA VRCV QYDG RRVV SGAY DFMV KVWD PETE TCLH TLQG HTNR VYSL QFDG IHVV SGSL DTSI RVWD VETG NCIH TLTG HQSL TSGM ELKD NILV SGNA DSTV KIWD IKTG QCLQ TLQG PNKH QSAV TCLQ FNKN FVIT SSDD GTVK LWDL KTGE FIRN LVTL ESGG SGGV VWRI RASN TKLV CAVG SRNG TEET KLLV LDFD VDMK
Gene ID:55294
Uniprot:Q969H0
Cellular Location:Cytoplasm, Nucleus, nucleolus, nucleoplasm
Calculated MW:66kDa/70kDa/79kDa
Observed MW:80kDa
Synonyms:FBXW7, AGO, CDC4, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4
Background:This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Code:Q969H0
NCBI GenInfo Identifier:44887885
NCBI Gene ID:55294
NCBI Accession:Q969H0.1
UniProt Secondary Accession:Q969H0,Q68DR0, Q96A16, Q96LE0, Q96RI2, Q9NUX6, B7ZLP9
UniProt Related Accession:Q969H0
Molecular Weight:707
NCBI Full Name:F-box/WD repeat-containing protein 7
NCBI Synonym Full Names:F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase
NCBI Official Symbol:FBXW7  
NCBI Official Synonym Symbols:AGO; CDC4; FBW6; FBW7; hAgo; FBX30; FBXW6; SEL10; hCdc4; FBXO30; SEL-10  
NCBI Protein Information:F-box/WD repeat-containing protein 7; archipelago; F-box protein FBW7; F-box protein FBX30; F-box protein SEL-10; homolog of C elegans sel-10; F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila)
UniProt Protein Name:F-box/WD repeat-containing protein 7
UniProt Synonym Protein Names:Archipelago homolog; hAgo; F-box and WD-40 domain-containing protein 7; F-box protein FBX30; SEL-10; hCdc4
Protein Family:Cdc42
UniProt Gene Name:FBXW7  
UniProt Entry Name:FBXW7_HUMAN
Anti-FBXW7 Antibody (CAB5872)Western blot analysis of extracts of various cell lines, using FBXW7 antibody (CAB5872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-FBXW7 Antibody (CAB5872)Immunohistochemistry of paraffin-embedded mouse lung using FBXW7 antibody (CAB5872) at dilution of 1:100 (40x lens).
Anti-FBXW7 Antibody (CAB5872)Immunohistochemistry of paraffin-embedded mouse kidney using FBXW7 antibody (CAB5872) at dilution of 1:100 (40x lens).
Anti-FBXW7 Antibody (CAB5872)Immunofluorescence analysis of U2OS cells using FBXW7 antibody (CAB5872) at dilution of 1:100. Blue: DAPI for nuclear staining.
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