Name: Anti-FBXW2 Antibody (CAB15996)
Brand: Signal Transduction Antibodies 2
SKU: CAB15996
Availability: OutOfStock

Antibody Name:	Anti-FBXW2 Antibody
Antibody SKU:	CAB15996
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human FBXW2 (NP_036296.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse kidney, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human FBXW2 (NP_036296.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MERK DFET WLDN ISVT FLSL TDLQ KNET LDHL ISLS GAVQ LRHL SNNL ETLL KRDF LKLL PLEL SFYL LKWL DPQT LLTC CLVS KQWN KVIS ACTE VWQT ACKN LGWQ IDDS VQDA LHWK KVYL KAIL RMKQ LEDH EAFE TSSL IGHS ARVY ALYY KDGL LCTG SDDL SAKL WDVS TGQC VYGI QTHT CAAV KFDE QKLV TGSF DNTV ACWE WSSG ARTQ HFRG HTGA VFSV DYND ELDI LVSG SADF TV
Gene ID:	26190
Uniprot:	Q9UKT8
Cellular Location:
Calculated MW:	44kDa/51kDa
Observed MW:	51kDa

Synonyms:

FBXW2, FBW2, Fwd2, Md6

Background:

F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats.

UniProt Protein Function:	FBXW2: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 9q34 Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: protein modification process; protein ubiquitination; proteolysis
NCBI Summary:	F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UKT8
NCBI GenInfo Identifier:	118402584
NCBI Gene ID:	26190
NCBI Accession:	NP_036296.2
UniProt Secondary Accession:	Q9UKT8,Q4VXH2, Q7Z4V6, Q8WV51, Q9HA09, Q9UKA3, B3KRL8
UniProt Related Accession:	Q9UKT8
Molecular Weight:	44,466 Da
NCBI Full Name:	F-box/WD repeat-containing protein 2
NCBI Synonym Full Names:	F-box and WD repeat domain containing 2
NCBI Official Symbol:	FBXW2
NCBI Official Synonym Symbols:	Md6; FBW2; Fwd2
NCBI Protein Information:	F-box/WD repeat-containing protein 2
UniProt Protein Name:	F-box/WD repeat-containing protein 2
UniProt Synonym Protein Names:	F-box and WD-40 domain-containing protein 2; Protein MD6
Protein Family:	F-box/WD repeat-containing protein
UniProt Gene Name:	FBXW2
UniProt Entry Name:	FBXW2_HUMAN

Western blot analysis of extracts of various cell lines, using FBXW2 antibody (CAB15996) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

FBXW2 Antibody (PACO09262)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)