Antibody Name:	Anti-FBXW11 Antibody
Antibody SKU:	CAB7784
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human FBXW11 (NP_387448.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, NIH/3T3, HepG2, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human FBXW11 (NP_387448.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEPD SVIE DKTI ELMN TSVM EDQN EDES PKKN TLWQ ISNG TSSV IVSR KRPS EGNY QKEK DLCI KYFD QWSE SDQV EFVE HLIS RMCH YQHG HINS YLKP MLQR DFIT ALPE QGLD HIAE NILS YLDA RSLC AAEL VCKE
Gene ID:	23291
Uniprot:	Q9UKB1
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	58kDa/60kDa/62kDa
Observed MW:	62kDa

Synonyms:

FBXW11, BTRC2, BTRCP2, FBW1B, FBXW1B, Fbw11, Hos

Background:

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.

UniProt Protein Function:	FBW1B: a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex May participate in Wnt signaling. Three alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 5q35.1 Cellular Component: centrosome; SCF ubiquitin ligase complex; nucleus; cytosol; ubiquitin ligase complex Molecular Function:protein dimerization activity; protein binding; ubiquitin-protein ligase activity Biological Process: proteasomal ubiquitin-dependent protein catabolic process; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; Wnt receptor signaling pathway; protein destabilization; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; rhythmic process; protein ubiquitination; positive regulation of circadian rhythm; negative regulation of NF-kappaB import into nucleus; protein amino acid dephosphorylation; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UKB1
NCBI GenInfo Identifier:	13124267
NCBI Gene ID:	23291
NCBI Accession:	Q9UKB1.1
UniProt Secondary Accession:	Q9UKB1,Q9P2S8, Q9P2S9, Q9Y4C6, B2RC98,
UniProt Related Accession:	Q9UKB1
Molecular Weight:	60,898 Da
NCBI Full Name:	F-box/WD repeat-containing protein 11
NCBI Synonym Full Names:	F-box and WD repeat domain containing 11
NCBI Official Symbol:	FBXW11
NCBI Official Synonym Symbols:	Hos; BTRC2; FBW1B; Fbw11; BTRCP2; FBXW1B
NCBI Protein Information:	F-box/WD repeat-containing protein 11; F-box and WD repeats protein beta-TrCP2; F-box and WD-40 domain protein 11; F-box and WD-40 domain protein 1B; F-box protein Fbw1b; F-box/WD repeat-containing protein 1B; beta-transducin repeat-containing protein 2; homologous to Slimb protein
UniProt Protein Name:	F-box/WD repeat-containing protein 11
UniProt Synonym Protein Names:	F-box and WD repeats protein beta-TrCP2; F-box/WD repeat-containing protein 1B; Homologous to Slimb protein; HOS
Protein Family:	F-box/WD repeat-containing protein
UniProt Gene Name:	FBXW11
UniProt Entry Name:	FBW1B_HUMAN

	Western blot analysis of extracts of various cell lines, using FBXW11 antibody (CAB7784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse lung using FBXW11 antibody (CAB7784) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using FBXW11 antibody (CAB7784).