Antibody Name:	Anti-FBXO7 Antibody
Antibody SKU:	CAB7464
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 353-522 of human FBXO7 (NP_036311.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Jurkat, Mouse testis, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 353-522 of human FBXO7 (NP_036311.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LSLS AVCR DLFT ASND PLLW RFLY LRDF RDNT VRVQ DTDW KELY RKRH IQRK ESPK GRFV MLLP SSTH TIPF YPNP LHPR PFPS SRLP PGII GGEY DQRP TLPY VGDP ISSL IPGP GETP SQFP PLRP RFDP VGPL PGPN PILP GRGG PNDR FPFR PSRG RPTD GRLS FM
Gene ID:	25793
Uniprot:	Q9Y3I1
Cellular Location:	Cytoplasm, Mitochondrion, Nucleus, cytosol
Calculated MW:	45kDa/49kDa/58kDa
Observed MW:	59kDa

Synonyms:

FBXO7, FBX, FBX07, FBX7, PARK15, PKPS

Background:

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it may play a role in regulation of hematopoiesis. Alternatively spliced transcript variants of this gene have been identified with the full-length natures of only some variants being determined.

UniProt Protein Function:	FBXO7: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes BIRC2 and DLGAP5. Defects in FBXO7 are the cause of Parkinson disease type 15 (PARK15); also known as parkinsonian-pyramidal syndrome. A neurodegenerative disorder characterized by parkinsonian and pyramidal signs. Clinical manifestations include tremor, bradykinesia, rigidity, postural instability, spasticity, mainly in the lower limbs, and hyperreflexia. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 22q12.3 Cellular Component: protein complex; mitochondrion; cytoplasm; nucleus; cytosol; ubiquitin ligase complex Molecular Function:protein binding; ubiquitin-protein ligase activity; protein kinase binding Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of cyclin-dependent protein kinase activity; mitochondrion degradation; regulation of protein stability; protein ubiquitination; protein targeting to mitochondrion; negative regulation of lymphocyte differentiation Disease: Parkinson Disease 15, Autosomal Recessive Early-onset
NCBI Summary:	This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it may play a role in regulation of hematopoiesis. Alternatively spliced transcript variants of this gene have been identified with the full-length natures of only some variants being determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y3I1
NCBI GenInfo Identifier:	13124249
NCBI Gene ID:	25793
NCBI Accession:	Q9Y3I1.1
UniProt Secondary Accession:	Q9Y3I1,Q5TGC4, Q5TI86, Q96HM6, Q9UF21, Q9UKT2, B4DNB3 B4DWX5,
UniProt Related Accession:	Q9Y3I1
Molecular Weight:	522
NCBI Full Name:	F-box only protein 7
NCBI Synonym Full Names:	F-box protein 7
NCBI Official Symbol:	FBXO7
NCBI Official Synonym Symbols:	FBX; FBX7; PKPS; FBX07; PARK15
NCBI Protein Information:	F-box only protein 7
UniProt Protein Name:	F-box only protein 7
Protein Family:	F-box only protein
UniProt Gene Name:	FBXO7
UniProt Entry Name:	FBX7_HUMAN

	Western blot analysis of extracts of various cell lines, using FBXO7 antibody (CAB7464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using FBXO7 antibody (CAB7464).