Signal Transduction Antibodies 3
Anti-FBXO4 Antibody (CAB9968)
(No reviews yet)
Write a Review
- SKU:
- CAB9968
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-FBXO4 Antibody |
| Antibody SKU: | CAB9968 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 138-387 of human FBXO4 (NP_036308.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:4000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SH-SY5Y, Mouse spleen, Mouse kidney, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 138-387 of human FBXO4 (NP_036308.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DYMA VYRM CCPY TRRA SKSS RPMY GAVT SFLH SLII QNEP RFAM FGPG LEEL NTSL VLSL MSSE ELCP TAGL PQRQ IDGI GSGV NFQL NNQH KFNI LILY STTR KERD RARE EHTS AVNK MFSR HNEG DDQQ GSRY SVIP QIQK VCEV VDGF IYVA NAEA HKRH EWQD EFSH IMAM TDPA FGSS GRPL LVLS CISQ GDVK RMPC FYLA HELH LNLL NHPW LVQD TEAE TLTG FLNG IEWI LEEV ESKR AR |
| Gene ID: | 26272 |
| Uniprot: | Q9UKT5 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 35kDa/44kDa |
| Observed MW: | 44kDa |
| Synonyms: | FBXO4, FBX4 |
| Background: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | FBXO4: Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1. Homodimer. Directly interacts with SKP1 and CUL1. Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO4) formed of CUL1, SKP1, RBX1 and FBXO4. Interacts with TERF1. Identified in a complex with CRYAB and CCND1. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 5p12 Cellular Component: cytosol; SCF ubiquitin ligase complex; ubiquitin ligase complex Molecular Function:protein binding; protein homodimerization activity; ubiquitin-protein ligase activity Biological Process: protein polyubiquitination; protein ubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process |
| NCBI Summary: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q9UKT5 |
| NCBI GenInfo Identifier: | 60416426 |
| NCBI Gene ID: | 26272 |
| NCBI Accession: | Q9UKT5.2 |
| UniProt Secondary Accession: | Q9UKT5,Q68CU8, Q86VT8, Q9UK98, |
| UniProt Related Accession: | Q9UKT5 |
| Molecular Weight: | 35,003 Da |
| NCBI Full Name: | F-box only protein 4 |
| NCBI Synonym Full Names: | F-box protein 4 |
| NCBI Official Symbol: | FBXO4 |
| NCBI Official Synonym Symbols: | FBX4 |
| NCBI Protein Information: | F-box only protein 4 |
| UniProt Protein Name: | F-box only protein 4 |
| Protein Family: | F-box only protein |
| UniProt Gene Name: | FBXO4 |
| UniProt Entry Name: | FBX4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FBXO4 antibody (CAB9968) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
View AllClose
Related Products
