Signal Transduction Antibodies 3

Anti-FBXO32 Antibody (CAB6825)

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SKU:
CAB6825
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-FBXO32 Antibody
Antibody SKU:CAB6825
Antibody Size:20uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide of human FBXO32
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:RD
Immunogen:A synthetic peptide of human FBXO32
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:114907
Uniprot:Q969P5
Cellular Location:Cytoplasm, Nucleus
Calculated MW:30kDa/41kDa
Observed MW:40KDa
Synonyms:FBXO32, Fbx32, MAFbx
Background:This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:FBXO32: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: nucleolus; Z disc

Molecular Function:protein binding

Biological Process: protein ubiquitination; response to denervation involved in regulation of muscle adaptation

NCBI Summary:This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
UniProt Code:Q969P5
NCBI GenInfo Identifier:20177894
NCBI Gene ID:114907
NCBI Accession:Q969P5.1
UniProt Secondary Accession:Q969P5,A4KYM0,
UniProt Related Accession:Q969P5
Molecular Weight:355
NCBI Full Name:F-box only protein 32
NCBI Synonym Full Names:F-box protein 32
NCBI Official Symbol:FBXO32  
NCBI Official Synonym Symbols:Fbx32; MAFbx  
NCBI Protein Information:F-box only protein 32; atrogin 1; atrogin-1; muscle atrophy F-box protein
UniProt Protein Name:F-box only protein 32
UniProt Synonym Protein Names:Atrogin-1; Muscle atrophy F-box protein; MAFbx
UniProt Gene Name:FBXO32  
UniProt Entry Name:FBX32_HUMAN
Anti-FBXO32 Antibody (CAB6825)Western blot analysis of extracts of RD cells, using FBXO32 antibody (CAB6825) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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