Signal Transduction Antibodies 2

Anti-FBXO32 Antibody (CAB3193)

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SKU:
CAB3193
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-FBXO32 Antibody
Antibody SKU:CAB3193
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 206-355 of human FBXO32 (NP_478136.1).
Application:WB IHC
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:Mouse, Rat
Positive Samples:Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 206-355 of human FBXO32 (NP_478136.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:WQQQ LNNI QITR PAFK GLTF TDLP LCLQ LNIM QRLS DGRD LVSL GQAA PDLH VLSE DRLL WKKL CQYH FSER QIRK RLIL SDKG QLDW KKMY FKLV RCYP RKEQ YGDT LQLC KHCH ILSW KGTD HPCT ANNP ESCS VSLS PQDF INLF KF
Gene ID:114907
Uniprot:Q969P5
Cellular Location:Cytoplasm, Nucleus
Calculated MW:27kDa/42kDa
Observed MW:40KDa
Synonyms:FBXO32, Fbx32, MAFbx
Background:This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:FBXO32: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: nucleolus; Z disc

Molecular Function:protein binding

Biological Process: protein ubiquitination; response to denervation involved in regulation of muscle adaptation

NCBI Summary:This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
UniProt Code:Q969P5
NCBI GenInfo Identifier:20177894
NCBI Gene ID:114907
NCBI Accession:Q969P5.1
UniProt Secondary Accession:Q969P5,A4KYM0,
UniProt Related Accession:Q969P5
Molecular Weight:355
NCBI Full Name:F-box only protein 32
NCBI Synonym Full Names:F-box protein 32
NCBI Official Symbol:FBXO32  
NCBI Official Synonym Symbols:Fbx32; MAFbx  
NCBI Protein Information:F-box only protein 32; atrogin 1; atrogin-1; muscle atrophy F-box protein
UniProt Protein Name:F-box only protein 32
UniProt Synonym Protein Names:Atrogin-1; Muscle atrophy F-box protein; MAFbx
UniProt Gene Name:FBXO32  
UniProt Entry Name:FBX32_HUMAN
Anti-FBXO32 Antibody (CAB3193)Western blot analysis of extracts of Mouse skeletal muscle, using FBXO32 antibody (CAB3193) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-FBXO32 Antibody (CAB3193)Immunohistochemistry of paraffin-embedded rat liver using FBXO32 antibody (CAB3193) at dilution of 1:100 (40x lens).
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