Cell Cycle Antibodies 1

Anti-FBXO31 Antibody (CAB12888)

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SKU:
CAB12888
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-FBXO31 Antibody
Antibody SKU:CAB12888
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 240-539 of human FBXO31 (NP_079011.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 240-539 of human FBXO31 (NP_079011.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GGRQ EEFR TWLR EEWG RTLE DIFH EHMQ ELIL MKFI YTSQ YDNC LTYR RIYL PPSR PDDL IKPG LFKG TYGS HGLE IVML SFHG RRAR GTKI TGDP NIPA GQQT VEID LRHR IQLP DLEN QRNF NELS RIVL EVRE RVRQ EQQE GGHE AGEG RGRQ GPRE SQPS PAQP RAEA PSKG PDGT PGED GGEP GDAV AAAE QPAQ CGQG QPFV LPVG VSSR NEDY PRTC RMCF YGTG LIAG HGFT SPER TPGV FILF DEDR FGFV WLEL KSFS LYSR VQAT FRNA DAPS PQAF DEML KNIQ SLTS
Gene ID:79791
Uniprot:Q5XUX0
Cellular Location:
Calculated MW:49kDa/60kDa
Observed MW:60kDa
Synonyms:FBXO31, FBX14, FBXO14, Fbx31, MRT45, pp2386
Background:This gene is a member of the F-box family. Members are classified into three classes according to the substrate interaction domain, FBW for WD40 repeats, FBL for leucing-rich repeats, and FBXO for other domains. This protein, classified into the last category because of the lack of a recognizable substrate binding domain, has been proposed to be a component of the SCF ubiquitination complex. It is thought to bind and recruit substrate for ubiquitination and degradation. This protein may have a role in regulating the cell cycle as well as dendrite growth and neuronal migration. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:FBXO31: Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor. Part of a SCF (SKP1-cullin-F-box) protein ligase complex. By DNA damage. Increases after UV irradiation, X-ray irradiation, oxidative stress (H(2)O(2)) or addition of the chemotherapeutic DNA-damaging agents etoposide, adriamycin, cisplatin or fluorouracil. Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow. Belongs to the FBXO31 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16q24.2

Cellular Component: cell soma; cytosol; SCF ubiquitin ligase complex

Molecular Function:cyclin binding; ubiquitin-protein ligase activity

Biological Process: cyclin catabolic process; G1 DNA damage checkpoint; protein polyubiquitination; response to DNA damage stimulus; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process

Disease: Mental Retardation, Autosomal Recessive 45

NCBI Summary:This gene is a member of the F-box family. Members are classified into three classes according to the substrate interaction domain, FBW for WD40 repeats, FBL for leucing-rich repeats, and FBXO for other domains. This protein, classified into the last category because of the lack of a recognizable substrate binding domain, has been proposed to be a component of the SCF ubiquitination complex. It is thought to bind and recruit substrate for ubiquitination and degradation. This protein may have a role in regulating the cell cycle as well as dendrite growth and neuronal migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
UniProt Code:Q5XUX0
NCBI GenInfo Identifier:146345419
NCBI Gene ID:79791
NCBI Accession:Q5XUX0.2
UniProt Secondary Accession:Q5XUX0,Q5K680, Q8WYV1, Q96D73, Q9UFV4,
UniProt Related Accession:Q5XUX0
Molecular Weight:49,039 Da
NCBI Full Name:F-box only protein 31
NCBI Synonym Full Names:F-box protein 31
NCBI Official Symbol:FBXO31  
NCBI Official Synonym Symbols:FBX14; Fbx31; MRT45; FBXO14; pp2386  
NCBI Protein Information:F-box only protein 31
UniProt Protein Name:F-box only protein 31
Protein Family:F-box only protein
UniProt Gene Name:FBXO31  
UniProt Entry Name:FBX31_HUMAN
Anti-FBXO31 Antibody (CAB12888)Western blot analysis of extracts of various cell lines, using FBXO31 antibody (CAB12888) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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