Signal Transduction Antibodies 2

Anti-FBXO25 Antibody (CAB15150)

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SKU:
CAB15150
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-FBXO25 Antibody
Antibody SKU:CAB15150
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 142-291 of human FBXO25 (NP_036305.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:DU145, 293T, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 142-291 of human FBXO25 (NP_036305.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:WQQQ LQDL QMTK QVNN GLTL SDLP LHML NNIL YRFS DGWD IITL GQVT PTLY MLSE DRQL WKKL CQYH FAEK QFCR HLIL SEKG HIEW KLMY FALQ KHYP AKEQ YGDT LHFC RHCS ILFW KDSG HPCT AADP DSCF TPVS PQHF IDLF KF
Gene ID:26260
Uniprot:Q8TCJ0
Cellular Location:Nucleus
Calculated MW:34kDa/42kDa/43kDa
Observed MW:22kDa
Synonyms:FBXO25, FBX25
Background:This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
UniProt Protein Function:FBXO25: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT). A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8p23.3

Cellular Component: nucleolus; nucleus; SCF ubiquitin ligase complex; ubiquitin ligase complex

Molecular Function:actin binding; protein binding; ubiquitin-protein ligase activity

Biological Process: protein ubiquitination

NCBI Summary:This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q8TCJ0
NCBI GenInfo Identifier:34878757
NCBI Gene ID:26260
NCBI Accession:NP_036305.2
UniProt Secondary Accession:Q8TCJ0,Q6PJ83, Q7Z4V4, Q9UKB8,
UniProt Related Accession:Q8TCJ0
Molecular Weight:
NCBI Full Name:F-box only protein 25 isoform 3
NCBI Synonym Full Names:F-box protein 25
NCBI Official Symbol:FBXO25  
NCBI Official Synonym Symbols:FBX25  
NCBI Protein Information:F-box only protein 25
UniProt Protein Name:F-box only protein 25
Protein Family:F-box only protein
UniProt Gene Name:FBXO25  
UniProt Entry Name:FBX25_HUMAN
Anti-FBXO25 Antibody (CAB15150)Western blot analysis of extracts of various cell lines, using FBXO25 antibody (CAB15150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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