Signal Transduction Antibodies 3

Anti-FBXO22 Antibody (CAB8367)

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SKU:
CAB8367

Description

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Antibody Name:Anti-FBXO22 Antibody
Antibody SKU:CAB8367
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 104-403 of human FBXO22 (NP_671717.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa, MCF-7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 104-403 of human FBXO22 (NP_671717.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YMAD SETF ISLE ECRG HKRA RKRT SMET ALAL EKLF PKQC QVLG IVTP GIVV TPMG SGSN RPQE IEIG ESGF ALLF PQIE GIKI QPFH FIKD PKNL TLER HQLT EVGL LDNP ELRV VLVF GYNC CKVG ASNY LQQV VSTF SDMN IILA GGQV DNLS SLTS EKNP LDID ASGV VGLS FSGH RIQS ATVL LNED VSDE KTAE AAMQ RLKA ANIP EHNT IGFM FACV GRGF QYYR AKGN VEAD AFRK FFPS VPLF GFFG NGEI GCDR IVTG NFIL RKCN EVKD DDLF HSYT TIMA LIHL GSSK
Gene ID:26263
Uniprot:Q8NEZ5
Cellular Location:Cytoplasm, Z line, myofibril, sarcomere
Calculated MW:4kDa/30kDa/44kDa
Observed MW:45kDa
Synonyms:FBXO22, FBX22, FISTC1
Background:This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and, as a transcriptional target of the tumor protein p53, is thought to be involved in degradation of specific proteins in response to p53 induction. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:FBXO22: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 15q24.2

Molecular Function:ubiquitin-protein ligase activity

Biological Process: protein modification process; ubiquitin-dependent protein catabolic process

NCBI Summary:This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and, as a transcriptional target of the tumor protein p53, is thought to be involved in degradation of specific proteins in response to p53 induction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
UniProt Code:Q8NEZ5
NCBI GenInfo Identifier:30580428
NCBI Gene ID:26263
NCBI Accession:Q8NEZ5.1
UniProt Secondary Accession:Q8NEZ5,Q0D2P8, Q6PIL5, Q8IXW3, Q9H824, Q9UKC0,
UniProt Related Accession:Q8NEZ5
Molecular Weight:30,589 Da
NCBI Full Name:F-box only protein 22
NCBI Synonym Full Names:F-box protein 22
NCBI Official Symbol:FBXO22  
NCBI Official Synonym Symbols:FBX22; FISTC1  
NCBI Protein Information:F-box only protein 22
UniProt Protein Name:F-box only protein 22
UniProt Synonym Protein Names:F-box protein FBX22p44
Protein Family:F-box only protein
UniProt Gene Name:FBXO22  
UniProt Entry Name:FBX22_HUMAN
Anti-FBXO22 Polyclonal Antibody (CAB8367)Western blot analysis of extracts of various cell lines, using FBXO22 antibody (CAB8367) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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Additional Information

Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
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