Signal Transduction Antibodies 2

Anti-FBXO21 Antibody (CAB16107)

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SKU:
CAB16107
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-FBXO21 Antibody
Antibody SKU:CAB16107
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 440-621 of human FBXO21 (NP_055817.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, MCF7, Mouse liver, Mouse testis, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 440-621 of human FBXO21 (NP_055817.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PEKV LDIL QHIQ TLDP GQHG AVGY LVQH TLEH IERK KEEV GVEV KLRS DEKH RDVC YSIG LIMK HKRY GYNC VIYG WDPT CMMG HEWI RNMN VHSL PHGH HQPF YNVL VEDG SCRY AAQE NLEY NVEP QEIS HPDV GRYF SEFT GTHY IPNA ELEI RYPE DLEF VYET VQNI YSAK KENI DE
Gene ID:23014
Uniprot:O94952
Cellular Location:
Calculated MW:71kDa/72kDa
Observed MW:72kDa
Synonyms:FBXO21, FBX21
Background:This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants.
UniProt Protein Function:FBXO21: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 12q24.22

NCBI Summary:This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
UniProt Code:O94952
NCBI GenInfo Identifier:218512038
NCBI Gene ID:23014
NCBI Accession:O94952.2
UniProt Secondary Accession:O94952,Q5BJG0, Q9H087, B3KMF0,
UniProt Related Accession:O94952
Molecular Weight:71,479 Da
NCBI Full Name:F-box only protein 21
NCBI Synonym Full Names:F-box protein 21
NCBI Official Symbol:FBXO21  
NCBI Official Synonym Symbols:FBX21  
NCBI Protein Information:F-box only protein 21
UniProt Protein Name:F-box only protein 21
Protein Family:F-box only protein
UniProt Gene Name:FBXO21  
UniProt Entry Name:FBX21_HUMAN
Anti-FBXO21 Antibody (CAB16107)Western blot analysis of extracts of various cell lines, using FBXO21 antibody (CAB16107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-FBXO21 Antibody (CAB16107)Immunohistochemistry of paraffin-embedded Mouse kidney using FBXO21 Rabbit pAb (CAB16107) at dilution of 1:100 (40x lens).
Anti-FBXO21 Antibody (CAB16107)Immunohistochemistry of paraffin-embedded Rat lung using FBXO21 Rabbit pAb (CAB16107) at dilution of 1:100 (40x lens).
Anti-FBXO21 Antibody (CAB16107)Immunohistochemistry of paraffin-embedded Human liver cancer using FBXO21 Rabbit pAb (CAB16107) at dilution of 1:100 (40x lens).
Anti-FBXO21 Antibody (CAB16107)Immunofluorescence analysis of L929 cells using FBXO21 antibody (CAB16107) at dilution of 1:100. Blue: DAPI for nuclear staining.
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