Antibody Name:	Anti-FBXO11 Antibody
Antibody SKU:	CAB6153
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 688-927 of human FBXO11 (NP_001177203.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:20 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HeLa, Mouse spleen, Mouse ovary, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 688-927 of human FBXO11 (NP_001177203.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GILV YNSG LGCI EDNE IFDN AMAG VWIK TDSN PTLR RNKI HDGR DGGI CIFN GGRG LLEE NDIF RNAQ AGVL ISTN SHPI LRKN RIFD GFAA GIEI TNHA TATL EGNQ IFNN RFGG LFLA SGVN VTMK DNKI MNNQ DAIE KAVS RGQC LYKI SSYT SYPM HDFY RCHT CNTT DRNA ICVN CIKK CHQG HDVE FIRH DRFF CDCG AGTL SNPC TLAG EPTH DTDT LYDS APPI ESNT LQHN
Gene ID:	80204
Uniprot:	Q86XK2
Cellular Location:	Chromosome, Nucleus
Calculated MW:	23kDa/62kDa/65kDa/94kDa/103kDa/106kDa
Observed MW:	130kDa

Synonyms:

FBXO11, FBX11, PRMT9, UBR6, UG063H01, VIT1

Background:

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.

UniProt Protein Function:	PRMT9: Substrate recognition component of the a (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Binds to and neddylates phosphorylated p53/TP53, inhibiting its transcriptional activity. SCF(FBXO11) does not seem to direct ubiquitination of TP53. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 2p16.3 Cellular Component: cytoplasm; nucleolus; chromosome; nucleus; ubiquitin ligase complex Molecular Function:protein binding; protein-arginine N-methyltransferase activity; zinc ion binding; ubiquitin-protein ligase activity Biological Process: ubiquitin-dependent protein catabolic process; sensory perception of sound; protein ubiquitination; protein modification process; peptidyl-arginine N-methylation
NCBI Summary:	This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2010]
UniProt Code:	Q86XK2
NCBI GenInfo Identifier:	124012093
NCBI Gene ID:	80204
NCBI Accession:	Q86XK2.3
UniProt Secondary Accession:	Q86XK2,Q52ZP1, Q53EP7, Q53RT5, Q8IXG3, Q96E90, Q9H6V8 Q9H9L1, Q9NR14, Q9UFK1, Q9UHI1, A1L491,
UniProt Related Accession:	Q86XK2
Molecular Weight:	927
NCBI Full Name:	F-box only protein 11
NCBI Synonym Full Names:	F-box protein 11
NCBI Official Symbol:	FBXO11
NCBI Official Synonym Symbols:	UBR6; VIT1; FBX11; PRMT9; UG063H01
NCBI Protein Information:	F-box only protein 11; vitiligo-associated protein 1; vitiligo-associated protein VIT-1; protein arginine N-methyltransferase 9; ubiquitin protein ligase E3 component n-recognin 6
UniProt Protein Name:	F-box only protein 11
UniProt Synonym Protein Names:	Protein arginine N-methyltransferase 9; Vitiligo-associated protein 1; VIT-1
Protein Family:	F-box only protein
UniProt Gene Name:	FBXO11
UniProt Entry Name:	FBX11_HUMAN

	Western blot analysis of extracts of various cell lines, using FBXO11 antibody (CAB6153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of MCF-7 cells using FBXO11 antibody (CAB6153). Blue: DAPI for nuclear staining.