Antibody Name:	Anti-FBXL5 Antibody
Antibody SKU:	CAB5602
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human FBXL5 (NP_036293.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Mouse eye, Mouse brain, Mouse lung, Rat testis, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human FBXL5 (NP_036293.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPF PEEV DVFT APHW RMKQ LVGL YCDK LSKT NFSN NNDF RALL QSLY ATFK EFKM HEQI ENEY IIGL LQQR SQTI YNVH SDNK LSEM LSLF EKGL KNVK NEYE QLNY AKQL KERL EAFT RDFL PHMK EEEE VFQP MLME YFTY EELK DIKK KVIA QHCS QKDT AELL RGLS LWNH AEER QKFF KYSV DEKS DKEA EVSE HSTG ITHL PPEV MLSI FSYL NPQE LCRC SQVS MKWS QLTK TGSL WKHL YPVH WARG DWYS GPAT ELDT EPDD EWVK NRKD ESRA FHEW DEDA DIDE SEES AEES IAIS IA
Gene ID:	26234
Uniprot:	Q9UKA1
Cellular Location:	Cytoplasm, perinuclear region
Calculated MW:	76kDa/78kDa
Observed MW:	79kDa

Synonyms:

FBXL5, FBL4, FBL5, FLR1

Background:

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. Alternatively spliced transcript variants have been described for this locus.

UniProt Protein Function:	FBXL5: Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 4p15.32 Cellular Component: perinuclear region of cytoplasm; SCF ubiquitin ligase complex Molecular Function:iron ion binding; protein binding Biological Process: iron ion homeostasis; protein ubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
NCBI Summary:	This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2010]
UniProt Code:	Q9UKA1
NCBI GenInfo Identifier:	37537864
NCBI Gene ID:	26234
NCBI Accession:	Q9UKA1.2
UniProt Secondary Accession:	Q9UKA1,Q4W5A8, Q8NHP3, Q9NXN2, Q9P0I0, Q9P0X5, Q9UJT7 Q9UKC8, A8MSK4, B4DIB5,
UniProt Related Accession:	Q9UKA1
Molecular Weight:	76,590 Da
NCBI Full Name:	F-box/LRR-repeat protein 5
NCBI Synonym Full Names:	F-box and leucine rich repeat protein 5
NCBI Official Symbol:	FBXL5
NCBI Official Synonym Symbols:	FBL4; FBL5; FLR1
NCBI Protein Information:	F-box/LRR-repeat protein 5
UniProt Protein Name:	F-box/LRR-repeat protein 5
UniProt Synonym Protein Names:	F-box and leucine-rich repeat protein 5; F-box protein FBL4/FBL5; p45SKP2-like protein
Protein Family:	F-box/LRR-repeat protein
UniProt Gene Name:	FBXL5
UniProt Entry Name:	FBXL5_HUMAN

	Western blot analysis of extracts of various cell lines, using FBXL5 antibody (CAB5602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human stomach using FBXL5 antibody (CAB5602) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using FBXL5 antibody (CAB5602) at dilution of 1:100 (40x lens).