Immunology Antibodies 1

Anti-FBXL2 Antibody (CAB10296)

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SKU:
CAB10296
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-FBXL2 Antibody
Antibody SKU:CAB10296
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-240 of human FBXL2 (NP_036289.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, A-549, HT-1080, SKOV3, Mouse brain, Mouse kidney, Rat brain, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-240 of human FBXL2 (NP_036289.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:STCY SLSR FCSK LKHL DLTS CVSI TNSS LKGI SEGC RNLE YLNL SWCD QITK DGIE ALVR GCRG LKAL LLRG CTQL EDEA LKHI QNYC HELV SLNL QSCS RITD EGVV QICR GCHR LQAL C
Gene ID:25827
Uniprot:Q9UKC9
Cellular Location:Lipid-anchor, Membrane
Calculated MW:39kDa/47kDa
Observed MW:38-47kDa
Synonyms:FBXL2, FBL2, FBL3
Background:This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:FBXL2: Calcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin. Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication.
UniProt Protein Details:

Protein type:Ubiquitin ligase

Chromosomal Location of Human Ortholog: 3p22.3

Cellular Component: cytoplasm; membrane; SCF ubiquitin ligase complex

Molecular Function:protein binding; protein phosphatase binding; ubiquitin-protein ligase activity

Biological Process: protein modification process; protein ubiquitination; proteolysis; regulation of autophagy; regulation of phosphoinositide 3-kinase cascade; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process

NCBI Summary:This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
UniProt Code:Q9UKC9
NCBI GenInfo Identifier:145559475
NCBI Gene ID:25827
NCBI Accession:Q9UKC9.3
UniProt Secondary Accession:Q9UKC9,Q6IAN3, Q9NVQ8, Q9UK27, Q9UKA5, Q9Y3Y9, B4DQV0 E9PD06,
UniProt Related Accession:Q9UKC9
Molecular Weight:47kDa
NCBI Full Name:F-box/LRR-repeat protein 2
NCBI Synonym Full Names:F-box and leucine rich repeat protein 2
NCBI Official Symbol:FBXL2  
NCBI Official Synonym Symbols:FBL2; FBL3  
NCBI Protein Information:F-box/LRR-repeat protein 2
UniProt Protein Name:F-box/LRR-repeat protein 2
UniProt Synonym Protein Names:F-box and leucine-rich repeat protein 2
Protein Family:F-box/LRR-repeat protein
UniProt Gene Name:FBXL2  
Anti-FBXL2 Antibody (CAB10296)Western blot analysis of extracts of various cell lines, using FBXL2 antibody (CAB10296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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