Immunology Antibodies 1
Anti-FBXL2 Antibody (CAB10296)
(No reviews yet)
Write a Review
- SKU:
- CAB10296
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-FBXL2 Antibody |
| Antibody SKU: | CAB10296 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-240 of human FBXL2 (NP_036289.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, A-549, HT-1080, SKOV3, Mouse brain, Mouse kidney, Rat brain, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-240 of human FBXL2 (NP_036289.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | STCY SLSR FCSK LKHL DLTS CVSI TNSS LKGI SEGC RNLE YLNL SWCD QITK DGIE ALVR GCRG LKAL LLRG CTQL EDEA LKHI QNYC HELV SLNL QSCS RITD EGVV QICR GCHR LQAL C |
| Gene ID: | 25827 |
| Uniprot: | Q9UKC9 |
| Cellular Location: | Lipid-anchor, Membrane |
| Calculated MW: | 39kDa/47kDa |
| Observed MW: | 38-47kDa |
| Synonyms: | FBXL2, FBL2, FBL3 |
| Background: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | FBXL2: Calcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin. Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase Chromosomal Location of Human Ortholog: 3p22.3 Cellular Component: cytoplasm; membrane; SCF ubiquitin ligase complex Molecular Function:protein binding; protein phosphatase binding; ubiquitin-protein ligase activity Biological Process: protein modification process; protein ubiquitination; proteolysis; regulation of autophagy; regulation of phosphoinositide 3-kinase cascade; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process |
| NCBI Summary: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010] |
| UniProt Code: | Q9UKC9 |
| NCBI GenInfo Identifier: | 145559475 |
| NCBI Gene ID: | 25827 |
| NCBI Accession: | Q9UKC9.3 |
| UniProt Secondary Accession: | Q9UKC9,Q6IAN3, Q9NVQ8, Q9UK27, Q9UKA5, Q9Y3Y9, B4DQV0 E9PD06, |
| UniProt Related Accession: | Q9UKC9 |
| Molecular Weight: | 47kDa |
| NCBI Full Name: | F-box/LRR-repeat protein 2 |
| NCBI Synonym Full Names: | F-box and leucine rich repeat protein 2 |
| NCBI Official Symbol: | FBXL2 |
| NCBI Official Synonym Symbols: | FBL2; FBL3 |
| NCBI Protein Information: | F-box/LRR-repeat protein 2 |
| UniProt Protein Name: | F-box/LRR-repeat protein 2 |
| UniProt Synonym Protein Names: | F-box and leucine-rich repeat protein 2 |
| Protein Family: | F-box/LRR-repeat protein |
| UniProt Gene Name: | FBXL2 |
 | Western blot analysis of extracts of various cell lines, using FBXL2 antibody (CAB10296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
View AllClose
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक