Name: Anti-FBP2 Antibody (CAB14077)
Brand: Metabolism Antibodies 1
SKU: CAB14077
Availability: OutOfStock

Antibody Name:	Anti-FBP2 Antibody
Antibody SKU:	CAB14077
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human FBP2 (NP_003828.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse skeletal muscle, Rat Skeletal muscle, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human FBP2 (NP_003828.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTDR SPFE TDML TLTR YVME KGRQ AKGT GELT QLLN SMLT AIKA ISSA VRKA GLAH LYGI AGSV NVTG DEVK KLDV LSNS LVIN MVQS SYST CVLV SEEN KDAI ITAK EKRG KYVV CFDP LDGS SNID CLAS IGTI FAIY RKTS EDEP SEKD ALQC GRNI VAAG YALY GSAT LVAL STGQ GVDL FMLD PALG EFVL VEKD VKIK KKGK IYSL NEGY AKYF DAAT TEYV QKKK FPED GSAP YGAR YVGS MVAD VHRT LVYG GIFL YPAN QKSP KGKL RLLY ECNP VAYI IEQA GGLA TTGT QPVL DVKP EAIH QRVP LILG SPED VQEY LTCV QKNQ AGS
Gene ID:	8789
Uniprot:	O00757
Cellular Location:	Cell junction, Cytoplasm, Nucleus, Z line, myofibril, sarcomere
Calculated MW:	36kDa
Observed MW:	36kDa

Synonyms:	FBP2
Background:	This gene encodes a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate.

UniProt Protein Function:	FBPase 2: a key enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a precursor to glucose 6-phosphate. A regulator of glucose synthesis from non-carbohydrates. Two paralogs of this enzyme exists in humans: FBP1 is expressed in the liver and gluconeogenic tissues, and FBP2 in striated muscle. FBP2 is only isozyme expressed in striated muscle and it is widely expressed in cells that are nongluconeogenic. Both forms are inhibited allosterically by AMP, NAD and Ca2+. The muscle form is about 100-fold more sensitive to AMP and NAD, and about 1000-fold more sensitive to inhibition by Ca2+, the major activator of glycolysis in striated muscle. Forms an active complex with aldolase that binds to the sarcomeric Z-line, which is disrupted by elevated calcium levels, blocking glycogen synthesis during exercise. Forms homotetramers that are stabilized in the active state by divalent cations (Mg2+, Mn2+ , Co+2, or Zn2+). Its interaction with aldolase appears to desensitizes it to inhibition by AMP and, partially, by Ca2+. Up to 50% of lactate generated in striated muscle is converted to glycogen.
UniProt Protein Details:	Protein type:EC 3.1.3.11; Carbohydrate Metabolism - pentose phosphate pathway; Phosphatase (non-protein); Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose Chromosomal Location of Human Ortholog: 9q22.3 Cellular Component: cytosol Molecular Function:fructose-bisphosphatase activity; protein binding Biological Process: fructose metabolic process; gluconeogenesis
NCBI Summary:	This gene encodes a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. [provided by RefSeq, Jul 2008]
UniProt Code:	O00757
NCBI GenInfo Identifier:	76789651
NCBI Gene ID:	8789
NCBI Accession:	O00757.2
UniProt Secondary Accession:	O00757,Q17R39, Q6FI53,
UniProt Related Accession:	O00757
Molecular Weight:	36,743 Da
NCBI Full Name:	Fructose-1,6-bisphosphatase isozyme 2
NCBI Synonym Full Names:	fructose-bisphosphatase 2
NCBI Official Symbol:	FBP2
NCBI Protein Information:	fructose-1,6-bisphosphatase isozyme 2
UniProt Protein Name:	Fructose-1,6-bisphosphatase isozyme 2
UniProt Synonym Protein Names:	D-fructose-1,6-bisphosphate 1-phosphohydrolase 2; Muscle FBPase
Protein Family:	Fat body protein
UniProt Gene Name:	FBP2
UniProt Entry Name:	F16P2_HUMAN

Western blot analysis of extracts of various cell lines, using FBP2 antibody (CAB14077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

Anti-FBP2 Antibody (CAB9850)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)