Antibody Name:	Anti-FBLN1 Antibody
Antibody SKU:	CAB16046
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 570-670 of human FBLN1 (NP_001987.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, A-549, U-87MG, Mouse brain, Rat brain, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 570-670 of human FBLN1 (NP_001987.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RLPC HENR ECSK LPLR ITYY HLSF PTNI QAPA VVFR MGPS SAVP GDSM QLAI TGGN EEGF FTTR KVSP HSGV VALT KPVP EPRD LLLT VKMD LSRH GTVS S
Gene ID:	2192
Uniprot:	P23142
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	61kDa/65kDa/74kDa/77kDa
Observed MW:	77KDa

Synonyms:

FBLN1, FBLN, FIBL1, fibulin-1

Background:

Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants.

UniProt Protein Function:	FBLN1: Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP. A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation. Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted; Extracellular matrix; Secreted, signal peptide Chromosomal Location of Human Ortholog: 22q13.31 Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane Molecular Function:protease activator activity; extracellular matrix structural constituent; calcium ion binding Biological Process: extracellular matrix organization and biogenesis; viral reproduction; embryo implantation Disease: Synpolydactyly 2
NCBI Summary:	Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants. [provided by RefSeq, Jul 2008]
UniProt Code:	P23142
NCBI GenInfo Identifier:	215274249
NCBI Gene ID:	2192
NCBI Accession:	P23142.4
UniProt Related Accession:	P23142
Molecular Weight:	Calculated: 61kDa; 65kDa; 74kDa; 77kDaObserved: 77kDa
NCBI Full Name:	Fibulin-1
NCBI Synonym Full Names:	fibulin 1
NCBI Official Symbol:	FBLN1
NCBI Official Synonym Symbols:	FBLN; FIBL1
NCBI Protein Information:	fibulin-1
UniProt Protein Name:	Fibulin-1
UniProt Gene Name:	FBLN1
UniProt Entry Name:	FBLN1_HUMAN

	Western blot analysis of extracts of various cell lines, using FBLN1 Rabbit pAb (CAB16046) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Western blot analysis of extracts of various cell lines, using FBLN1 Rabbit pAb (CAB16046) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.