Name: Anti-FBLIM1 Antibody (CAB15850)
Brand: Cell Biology Antibodies 6
SKU: CAB15850
Availability: OutOfStock

Antibody Name:	Anti-FBLIM1 Antibody
Antibody SKU:	CAB15850
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 180-272 of human FBLIM1 (NP_060026.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 180-272 of human FBLIM1 (NP_060026.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TDIC AFCH KTVS PREL AVEA MKRQ YHAQ CFTC RTCR RQLA GQSF YQKD GRPL CEPC YQDT LERC GKCG EVVR DHII RALG QAFH PSCF TCVT C
Gene ID:	54751
Uniprot:	Q8WUP2
Cellular Location:	Cell junction, Cytoplasm, cell cortex, cytoskeleton, focal adhesion
Calculated MW:	30kDa/40kDa
Observed MW:	41kDa

Synonyms:

FBLIM1, CAL, FBLP-1, FBLP1

Background:

This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	FBLIM1: Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin- containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1p36.21 Cellular Component: cytosol; focal adhesion; stress fiber Molecular Function:filamin binding; protein binding Biological Process: cell-cell adhesion; regulation of integrin activation
NCBI Summary:	This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8WUP2
NCBI GenInfo Identifier:	125987829
NCBI Gene ID:	54751
NCBI Accession:	Q8WUP2.2
UniProt Secondary Accession:	Q8WUP2,Q5VVE0, Q5VVE1, Q8IX23, Q96T00, Q9UFD6, B3KNY0
UniProt Related Accession:	Q8WUP2
Molecular Weight:	30,770 Da
NCBI Full Name:	Filamin-binding LIM protein 1
NCBI Synonym Full Names:	filamin binding LIM protein 1
NCBI Official Symbol:	FBLIM1
NCBI Official Synonym Symbols:	CAL; FBLP1; FBLP-1
NCBI Protein Information:	filamin-binding LIM protein 1
UniProt Protein Name:	Filamin-binding LIM protein 1
UniProt Synonym Protein Names:	Migfilin; Mitogen-inducible 2-interacting protein; MIG2-interacting protein
Protein Family:	Filamin-binding LIM protein
UniProt Gene Name:	FBLIM1
UniProt Entry Name:	FBLI1_HUMAN

Western blot analysis of extracts of rat heart, using FBLIM1 antibody (CAB15850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

FBLIM1 Antibody (PACO09241)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)