Metabolism Antibodies 1

Anti-Fatty Acid Synthase Antibody (CAB0461)

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SKU:
CAB0461
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-Fatty Acid Synthase Antibody
Antibody SKU:CAB0461
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (NP_004095.4).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:Mouse liver, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (NP_004095.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QQQT QLNL RSLL VNPE GPTL MRLN SVQS SERP LFLV HPIE GSTT VFHS LASR LSIP TYGL QCTR AAPL DSIH SLAA YYID CIRQ VQPE GPYR VAGY SYGA CVAF EMCS QLQA QQSP APTH NSLF LFDG SPTY VLAY TQSY RAKL TPGC EAEA ETEA ICFF VQQF TDME HNRV LEAL LPLK GLEE RVAA AVDL IIKS HQGL DRQE LSFA ARSF YYKL RAAE QYTP KAKY HGNV MLLR AKTG GAYG EDLG ADYN LSQV CDGK VSVH VIEG DHRT LLEG SGLE SIIS IIHS SLAE PRVS VREG
Gene ID:2194
Uniprot:P49327
Cellular Location:Cytoplasm, Melanosome
Calculated MW:273kDa
Observed MW:273kDa
Synonyms:FAS, OA-519, SDR27X1, FASN
Background:The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
UniProt Protein Function:FASN: a multifunctional enzyme. Catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
UniProt Protein Details:

Protein type:EC 1.3.1.39; EC 1.1.1.100; EC 2.3.1.39; EC 3.1.2.14; Hydrolase; EC 2.3.1.41; EC 4.2.1.59; Oxidoreductase; EC 2.3.1.38; Lyase; EC 2.3.1.85; Lipid Metabolism - fatty acid biosynthesis; Acetyltransferase

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: Golgi apparatus; membrane; glycogen granule; mitochondrion; cytoplasm; plasma membrane; melanosome; cytosol

Molecular Function:fatty-acid synthase activity; protein homodimerization activity; myristoyl-[acyl-carrier-protein] hydrolase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; zinc ion binding; enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; drug binding; [acyl-carrier-protein] S-acetyltransferase activity; 3-oxoacyl-[acyl-carrier-protein] reductase activity; protein binding; 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; oleoyl-[acyl-carrier-protein] hydrolase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; [acyl-carrier-protein] S-malonyltransferase activity; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity

Biological Process: osteoblast differentiation; vitamin metabolic process; acetyl-CoA metabolic process; pantothenate metabolic process; energy reserve metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid metabolic process; fatty acid biosynthetic process; water-soluble vitamin metabolic process

NCBI Summary:The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]
UniProt Code:P49327
NCBI GenInfo Identifier:269849686
NCBI Gene ID:2194
NCBI Accession:P49327.3
UniProt Secondary Accession:P49327,Q13479, Q16702, Q4LE83, Q6P4U5, Q6SS02, Q969R1 Q96C68, Q96IT0,
UniProt Related Accession:P49327
Molecular Weight:2511
NCBI Full Name:Fatty acid synthase
NCBI Synonym Full Names:fatty acid synthase
NCBI Official Symbol:FASN  
NCBI Official Synonym Symbols:FAS; OA-519; SDR27X1  
NCBI Protein Information:fatty acid synthase; short chain dehydrogenase/reductase family 27X, member 1
UniProt Protein Name:Fatty acid synthase
Protein Family:Fatty acid synthase
UniProt Gene Name:FASN  
UniProt Entry Name:FAS_HUMAN
Anti-Fatty Acid Synthase Antibody (CAB0461)Western blot analysis of extracts of various cell lines, using Fatty Acid Synthase antibody (CAB0461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-Fatty Acid Synthase Antibody (CAB0461)Immunohistochemistry of paraffin-embedded human breast cancer using Fatty Acid Synthase antibody (CAB0461) at dilution of 1:200 (40x lens).
Anti-Fatty Acid Synthase Antibody (CAB0461)Immunofluorescence analysis of A549 cells using Fatty Acid Synthase antibody (CAB0461). Blue: DAPI for nuclear staining.
Anti-Fatty Acid Synthase Antibody (CAB0461)Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Fatty Acid Synthase antibody (CAB0461). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase antibody (CAB0461) at a dilition of 1:1000.
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