Cell Death Antibodies 2

Anti-FASTK Antibody (CAB7069)

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SKU:
CAB7069
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-FASTK Antibody
Antibody SKU:CAB7069
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 360-549 of human FASTK (NP_006703.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:NCI-H460, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 360-549 of human FASTK (NP_006703.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LLDT AVEL ELPG YRGP RLPR RQQV PIFP QPLI TDRA RCKY SHKD IVAE GLRQ LLGE EKYR QDLT VPPG YCTD FLLC ASSS GAVL PVRT QDPF LPYP PRSC PQGQ AASS ATTR DPAQ RVVL VLRE RWHF CRDG RVLL GSRA LRER HLGL MGYQ LLPL PFEE LESQ RGLP QLKS YLRQ KLQA LGLR WGPE GG
Gene ID:10922
Uniprot:Q14296
Cellular Location:
Calculated MW:45kDa/57kDa/58kDa/61kDa
Observed MW:61kDa
Synonyms:FASTK, FAST
Background:The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined.
UniProt Protein Function:FASTK: an atypical protein kinase. Fas-mediated apoptosis is characterized by FASTK- dephosphorylation and TIA-1-phosphorylation. Both are activated and FASTK-mediated TIA-1 activation play a key role in apoptosis.
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, atypical; EC 2.7.11.8; ATYPICAL group; FAST family

Chromosomal Location of Human Ortholog: 7q35

Molecular Function:protein binding; protein serine/threonine kinase activity

Biological Process: protein amino acid phosphorylation; regulation of RNA splicing

NCBI Summary:The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined. [provided by RefSeq, Jul 2008]
UniProt Code:Q14296
NCBI GenInfo Identifier:13124202
NCBI Gene ID:10922
NCBI Accession:Q14296.1
UniProt Secondary Accession:Q14296,Q59EM8, Q8IVA0, A8K867, F8VTW9,
UniProt Related Accession:Q14296
Molecular Weight:57,576 Da
NCBI Full Name:Fas-activated serine/threonine kinase
NCBI Synonym Full Names:Fas activated serine/threonine kinase
NCBI Official Symbol:FASTK  
NCBI Official Synonym Symbols:FAST  
NCBI Protein Information:fas-activated serine/threonine kinase
UniProt Protein Name:Fas-activated serine/threonine kinase
Protein Family:Fas-activated serine/threonine kinase
UniProt Gene Name:FASTK  
UniProt Entry Name:FASTK_HUMAN
Anti-FASTK Antibody (CAB7069)Western blot analysis of extracts of various cell lines, using FASTK antibody (CAB7069) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-FASTK Antibody (CAB7069)Immunohistochemistry of paraffin-embedded rat liver using FASTK antibody (CAB7069) at dilution of 1:100 (40x lens).
Anti-FASTK Antibody (CAB7069)Immunohistochemistry of paraffin-embedded human kidney using FASTK antibody (CAB7069) at dilution of 1:100 (40x lens).
Anti-FASTK Antibody (CAB7069)Immunofluorescence analysis of U2OS cells using FASTK antibody (CAB7069). Blue: DAPI for nuclear staining.
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