Cell Death Antibodies 1

Anti-FasLG Antibody (CAB0234)

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SKU:
CAB0234
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-FasLG Antibody
Antibody SKU:CAB0234
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human FasLG (NP_000630.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse heart
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human FasLG (NP_000630.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GMFQ LFHL QKEL AELR ESTS QMHT ASSL EKQI GHPS PPPE KKEL RKVA HLTG KSNS RSMP LEWE DTYG IVLL SGVK YKKG GLVI NETG LYFV YSKV YFRG Q
Gene ID:356
Uniprot:P48023
Cellular Location:Cell membrane, Cytoplasmic vesicle lumen, Lysosome lumen, Nucleus, Secreted, Single-pass type II membrane protein
Calculated MW:14kDa/31kDa
Observed MW:46kDa
Synonyms:FASLG, ALPS1B, APT1LG1, APTL, CD178, CD95-L, CD95L, FASL, TNFSF6, TNLG1A, Fas ligand, FasL, FasLG
Background:This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described.
UniProt Protein Function:FasL: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Apoptosis; Cytokine

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: extracellular space; lysosomal lumen; perinuclear region of cytoplasm; integral to plasma membrane; extracellular region; plasma membrane; caveola; nucleus; external side of plasma membrane

Molecular Function:protein binding; death receptor binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; signal transduction; cellular chloride ion homeostasis; endosomal lumen acidification; inflammatory cell apoptosis; negative regulation of angiogenesis; induction of apoptosis via death domain receptors; cell-cell signaling; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of neuron apoptosis; positive regulation of cell proliferation; immune response; retinal cell programmed cell death

Disease: Lung Cancer; Autoimmune Lymphoproliferative Syndrome

NCBI Summary:This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]
UniProt Code:P48023
NCBI GenInfo Identifier:1345957
NCBI Gene ID:356
NCBI Accession:P48023.1
UniProt Secondary Accession:P48023,Q9BZP9,
UniProt Related Accession:P48023
Molecular Weight:14,006 Da
NCBI Full Name:Tumor necrosis factor ligand superfamily member 6
NCBI Synonym Full Names:Fas ligand (TNF superfamily, member 6)
NCBI Official Symbol:FASLG  
NCBI Official Synonym Symbols:APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1  
NCBI Protein Information:tumor necrosis factor ligand superfamily member 6; CD95 ligand; fas antigen ligand; apoptosis antigen ligand; apoptosis (APO-1) antigen ligand 1
UniProt Protein Name:Tumor necrosis factor ligand superfamily member 6
UniProt Synonym Protein Names:Apoptosis antigen ligand; APTL; CD95 ligand; CD95-L; Fas antigen ligand; Fas ligand; FasL; CD_antigen: CD178Cleaved into the following 4 chains:Tumor necrosis factor ligand superfamily member 6, membrane form; Tumor necrosis factor ligand superfamily member 6, soluble formAlternative name(s):Receptor-binding FasL ectodomain; Soluble Fas ligand; sFasL
Protein Family:S-fimbrial adhesin protein
UniProt Gene Name:FASLG  
UniProt Entry Name:TNFL6_HUMAN
Anti-FasLG Antibody (CAB0234)Western blot analysis of extracts of Mouse heart, using FasLG antibody (CAB0234) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5min.
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