Metabolism Antibodies 1

Anti-FARSB Antibody (CAB15375)

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SKU:
CAB15375
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-FARSB Antibody
Antibody SKU:CAB15375
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 240-589 of human FARSB (NP_005678.3).
Application:WB IF
Recommended Dilution:WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, HeLa, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 240-589 of human FARSB (NP_005678.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DHSR ITVN TRNI FIEC TGTD FTKA KIVL DIIV TMFS EYCE NQFT VEAA EVVF PNGK SHTF PELA YRKE MVRA DLIN KKVG IRET PENL AKLL TRMY LKSE VIGD GNQI EIEI PPTR ADII HACD IVED AAIA YGYN NIQM TLPK TYTI ANQF PLNK LTEL LRHD MAAA GFTE ALTF ALCS QEDI ADKL GVDI SATK AVHI SNPK TAEF QVAR TTLL PGLL KTIA ANRK MPLP LKLF EISD IVIK DSNT DVGA KNYR HLCA VYYN KNPG FEII HGLL DRIM QLLD VPPG EDKG GYVI KASE GPAF FPGR CAEI FARG QSVG KLGV LHPD VITK FELT MPCS SLEI NVGP FL
Gene ID:10056
Uniprot:Q9NSD9
Cellular Location:Cytoplasm
Calculated MW:54kDa/66kDa
Observed MW:69kDa
Synonyms:FARSB, FARSLB, FRSB, HSPC173, PheHB, PheRS
Background:This gene encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:FARSLB: a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Protein type:Ligase; EC 6.1.1.20; Translation

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function:RNA binding; phenylalanine-tRNA ligase activity; magnesium ion binding; ATP binding

Biological Process: tRNA aminoacylation for protein translation; translation; phenylalanyl-tRNA aminoacylation; gene expression

NCBI Summary:This gene encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
UniProt Code:Q9NSD9
NCBI GenInfo Identifier:296452943
NCBI Gene ID:10056
NCBI Accession:Q9NSD9.3
UniProt Related Accession:Q9NSD9
Molecular Weight:
NCBI Full Name:Phenylalanine--tRNA ligase beta subunit
NCBI Synonym Full Names:phenylalanyl-tRNA synthetase subunit beta
NCBI Official Symbol:FARSB  
NCBI Official Synonym Symbols:FRSB; PheHB; PheRS; FARSLB; RILDBC; HSPC173; NEDBLLA  
NCBI Protein Information:phenylalanine--tRNA ligase beta subunit
UniProt Protein Name:Phenylalanine--tRNA ligase beta subunit
UniProt Synonym Protein Names:Phenylalanyl-tRNA synthetase beta subunit; PheRS
UniProt Gene Name:FARSB  
UniProt Entry Name:SYFB_HUMAN
Anti-FARSB Antibody (CAB15375)Western blot analysis of extracts of various cell lines, using FARSB antibody (CAB15375) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-FARSB Antibody (CAB15375)Immunofluorescence analysis of C6 cells using FARSB antibody (CAB15375) at dilution of 1:100. Blue: DAPI for nuclear staining.
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