Cell Cycle Antibodies 1

Anti-FANCI Antibody (CAB3447)

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SKU:
CAB3447
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-FANCI Antibody
Antibody SKU:CAB3447
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1061-1328 of human FANCI (NP_001106849.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa, 293T, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1061-1328 of human FANCI (NP_001106849.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DQDV EVEK TNHF AIVN LRTA APTV CLLV LSQA EKVL EEVD WLIT KLKG QVSQ ETLS EEAS SQAT LPNQ PVEK AIIM QLGT LLTF FHEL VQTA LPSG SCVD TLLK DLCK MYTT LTAL VRYY LQVC QSSG GIPK NMEK LVKL SGSH LTPL CYSF ISYV QNKS KSLN YTGE KKEK PAAV ATAM ARVL RETK PIPN LIFA IEQY EKFL IHLS KKSK VNLM QHMK LSTS RDFK IKGN ILDM VLRE DGED ENEE GTAS EHGG QNKE PAKK KRKK
Gene ID:55215
Uniprot:Q9NVI1
Cellular Location:Nucleus
Calculated MW:27kDa/142kDa/149kDa
Observed MW:149kDa
Synonyms:FANCI, KIAA1794
Background:The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group I. Alternative splicing results in two transcript variants encoding different isoforms.
UniProt Protein Function:FANCI: Plays an essential role in the repair of DNA double- strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single- stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage. Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI). A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA repair, damage; Cell cycle regulation

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: nucleoplasm; membrane; cytoplasm; plasma membrane; nucleus

Molecular Function:protein binding; DNA binding

Biological Process: positive regulation of protein ubiquitination; cell cycle; DNA repair

Disease: Fanconi Anemia, Complementation Group I; Tracheoesophageal Fistula With Or Without Esophageal Atresia

NCBI Summary:The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group I. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:Q9NVI1
NCBI GenInfo Identifier:212276518
NCBI Gene ID:55215
NCBI Accession:Q9NVI1.4
UniProt Related Accession:Q9NVI1
Molecular Weight:~ 149kDa
NCBI Full Name:Fanconi anemia group I protein
NCBI Synonym Full Names:FA complementation group I
NCBI Official Symbol:FANCI  
NCBI Official Synonym Symbols:KIAA1794  
NCBI Protein Information:Fanconi anemia group I protein
UniProt Protein Name:Fanconi anemia group I protein
Protein Family:Fanconi anemia group I protein
UniProt Gene Name:FANCI  
UniProt Entry Name:FANCI_HUMAN
Anti-FANCI Antibody (CAB3447)Western blot analysis of extracts of various cell lines, using FANCI antibody (CAB3447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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