KO Validated Antibodies 1

Anti-FANCD2 Antibody (CAB18055)[KO Validated]

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SKU:
CAB18055
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-FANCD2 Antibody [KO Validated]
Antibody SKU:CAB18055
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human FANCD2 (NP_149075.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human FANCD2 (NP_149075.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVSK RRLS KSED KESL TEDA SKTR KQPL SKKT KKSH IANE VEEN DSIF VKLL KISG IILK TGES QNQL AVDQ IAFQ KKLF QTLR RHPS YPKI IEEF VSGL ESYI EDED SFRN CLLS CERL QDEE ASMG ASYS KSLI KLLL GIDI LQPA IIKT LFEK LPEY FFEN KNSD EINI PRLI VSQL KWLD RVVD GKDL TTKI MQLI SIAP ENLQ HDII TSLP EILG DSQH ADVG KE
Gene ID:2177
Uniprot:Q9BXW9
Cellular Location:Nucleus
Calculated MW:27kDa/140kDa/164kDa/166kDa
Observed MW:166kDa
Anti-FANCD2 Antibody [KO Validated] (CAB18055)Western blot analysis of extracts from normal (control) and FANCD2 knockout (KO) HeLa cells, using FANCD2 antibody (CAB18055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Synonyms:FA4, FAD, FACD, FAD2, FA-D2, FANCD, FANCD2
Background:The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:FANCD2: Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching. Interacts directly with FANCE and FANCI. Interacts with USP1 and MEN1. The ubiquitinated form specifically interacts with BRCA1 and BLM. Both the nonubiquitinated and the monoubiquitinated forms interact with BRCA2; this interaction is mediated by phosphorylated FANCG and the complex also includes XCCR3. The ubiquitinated form specifically interacts with MTMR15/FAN1 (via UBZ-type zinc finger), leading to recruit MTMR15/FAN1 to sites of DNA damage. Interacts with DCLRE1B/Apollo. Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas. Highly expressed in testis, where expression is restricted to maturing spermatocytes. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA repair, damage

Chromosomal Location of Human Ortholog: 3p26

Cellular Component: condensed chromosome; cytoplasm; nucleolus; nucleoplasm; nucleus

Molecular Function:protein binding

Biological Process: DNA repair; gamete generation; response to gamma radiation; synapsis

Disease: Fanconi Anemia, Complementation Group D2; Tracheoesophageal Fistula With Or Without Esophageal Atresia

NCBI Summary:The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
UniProt Code:Q9BXW9
NCBI GenInfo Identifier:727863575
NCBI Gene ID:2177
NCBI Accession:Q9BXW9.2
UniProt Secondary Accession:Q9BXW9,Q2LA86, Q69YP9, Q6PJN7, Q9BQ06, Q9H9T9,
UniProt Related Accession:Q9BXW9
Molecular Weight:27,501 Da
NCBI Full Name:Fanconi anemia group D2 protein
NCBI Synonym Full Names:Fanconi anemia complementation group D2
NCBI Official Symbol:FANCD2  
NCBI Official Synonym Symbols:FA4; FAD; FACD; FAD2; FA-D2; FANCD  
NCBI Protein Information:Fanconi anemia group D2 protein
UniProt Protein Name:Fanconi anemia group D2 protein
Protein Family:FANCD2 opposite strand protein
UniProt Gene Name:FANCD2  
UniProt Entry Name:FACD2_HUMAN
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