Cell Biology Antibodies 6

Anti-FADS1 Antibody (CAB16306)

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SKU:
CAB16306
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-FADS1 Antibody
Antibody SKU:CAB16306
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human FADS1 (NP_037534.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, Mouse testis, Rat lung
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human FADS1 (NP_037534.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YNIS EFTR RHPG GSRV ISHY AGQD ATDP FVAF HINK GLVK KYMN SLLI GELS PEQP SFEP TKNK ELTD EFRE LRAT VERM GLMK ANHV FFLL YLLH ILLL D
Gene ID:3992
Uniprot:O60427
Cellular Location:
Calculated MW:42kDa/51kDa
Observed MW:52kDa
Synonyms:FADS1, D5D, FADS6, FADSD5, LLCDL1, TU12
Background:The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.
UniProt Protein Function:FADS1: Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the desaturation of dihomo-gamma-linoleic acid (DHGLA) (20:3n-6) and eicosatetraenoic acid (20:4n-3) to generate arachidonic acid (AA) (20:4n-6) and eicosapentaenoic acid (EPA)(20:5n-3) respectively. Belongs to the fatty acid desaturase family.
UniProt Protein Details:

Protein type:EC 1.14.19.-; Lipid Metabolism - unsaturated fatty acid biosynthesis; Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: endoplasmic reticulum membrane; membrane

Molecular Function:C-5 sterol desaturase activity; omega-6 fatty acid desaturase activity; oxidoreductase activity

Biological Process: cellular response to starvation; icosanoid biosynthetic process; linoleic acid metabolic process; phospholipid biosynthetic process; unsaturated fatty acid biosynthetic process

NCBI Summary:The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]
UniProt Code:O60427
NCBI GenInfo Identifier:565683215
NCBI Gene ID:3992
NCBI Accession:O60427.3
UniProt Secondary Accession:O60427,Q53GM5, Q8N3A6, Q8NCC7, Q8NCG0, Q96I39, Q96SV3 Q96T10, Q9NRP8, Q9NYX1, A8K0I7, B2RAI0,
UniProt Related Accession:O60427
Molecular Weight:42,474 Da
NCBI Full Name:Fatty acid desaturase 1
NCBI Synonym Full Names:fatty acid desaturase 1
NCBI Official Symbol:FADS1  
NCBI Official Synonym Symbols:D5D; TU12; FADS6; FADSD5; LLCDL1  
NCBI Protein Information:fatty acid desaturase 1
UniProt Protein Name:Fatty acid desaturase 1
UniProt Synonym Protein Names:Delta(5) fatty acid desaturase; D5D; Delta(5) desaturase; Delta-5 desaturase
Protein Family:Fatty acid desaturase
UniProt Gene Name:FADS1  
Anti-FADS1 Antibody (CAB16306)Western blot analysis of extracts of various cell lines, using FADS1 antibody (CAB16306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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