KO Validated Antibodies 1

Anti-FADD Antibody (CAB18082)[KO Validated]

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SKU:
CAB18082
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-FADD Antibody [KO Validated]
Antibody SKU:CAB18082
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-208 of human FADD (NP_003815.1).
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-208 of human FADD (NP_003815.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDPF LVLL HSVS SSLS SSEL TELK FLCL GRVG KRKL ERVQ SGLD LFSM LLEQ NDLE PGHT ELLR ELLA SLRR HDLL RRVD DFEA GAAA GAAP GEED LCAA FNVI CDNV GKDW RRLA RQLK VSDT KIDS IEDR YPRN LTER VRES LRIW KNTE KENA TVAH LVGA LRSC QMNL VADL VQEV QQAR DLQN RSGA MSPM SWNS DAST SEAS
Gene ID:8772
Uniprot:Q13158
Cellular Location:
Calculated MW:23kDa
Observed MW:28kDa
Anti-FADD Antibody [KO Validated] (CAB18082)Western blot analysis of extracts from normal (control) and FADD knockout (KO) HeLa cells, using FADD antibody (CAB18082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Synonyms:GIG3, MORT1, FADD, FADD
Background:The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
UniProt Protein Function:FADD: an adaptor molecule that mediates apoptosis. Recruited through its C-terminal death domain by Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TRAIL-receptor, participating in the death signaling initiated by these receptors. Interaction with the receptors unmasks the N-terminal effector domain of this protein, which recruits caspase-8, and thereby activates the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
UniProt Protein Details:

Protein type:Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: neuron projection; CD95 death-inducing signaling complex; cytosol; lipid raft

Molecular Function:identical protein binding; protein binding; protease binding; death receptor binding; protein complex binding; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: viral reproduction; positive regulation of apoptosis; apoptosis; positive regulation of T cell mediated cytotoxicity; positive regulation of proteolysis; protein heterooligomerization; T cell differentiation in the thymus; toll-like receptor 3 signaling pathway; positive regulation of activated T cell proliferation; positive regulation of interleukin-8 production; T cell homeostasis; positive regulation of macrophage differentiation; toll-like receptor 4 signaling pathway; defense response to virus; positive regulation of adaptive immune response; spleen development; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; thymus development; MyD88-independent toll-like receptor signaling pathway; positive regulation of tumor necrosis factor production; lymph node development; positive regulation of interferon-gamma production; induction of apoptosis via death domain receptors; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter

Disease: Infections, Recurrent, With Encephalopathy, Hepatic Dysfunction, And Cardiovascular Malformations

NCBI Summary:The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]
UniProt Code:Q13158
NCBI GenInfo Identifier:2498355
NCBI Gene ID:8772
NCBI Accession:Q13158.1
UniProt Secondary Accession:Q13158,Q14866, Q6IBR4,
UniProt Related Accession:Q13158
Molecular Weight:208
NCBI Full Name:FAS-associated death domain protein
NCBI Synonym Full Names:Fas (TNFRSF6)-associated via death domain
NCBI Official Symbol:FADD  
NCBI Official Synonym Symbols:GIG3; MORT1  
NCBI Protein Information:FAS-associated death domain protein; growth-inhibiting gene 3 protein; mediator of receptor induced toxicity; mediator of receptor-induced toxicity; Fas-associating protein with death domain; Fas-associating death domain-containing protein
UniProt Protein Name:FAS-associated death domain protein
UniProt Synonym Protein Names:FAS-associating death domain-containing protein; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity; Protein FADD
Protein Family:Fas-associated death domain protein
UniProt Gene Name:FADD  
UniProt Entry Name:FADD_HUMAN
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