Name: Anti-FADD Antibody (CAB18044)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB18044
Availability: OutOfStock

Antibody Name:	Anti-FADD Antibody [KO Validated]
Antibody SKU:	CAB18044
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human FADD

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant protein of human FADD
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	8772
Uniprot:	Q13158
Cellular Location:
Calculated MW:	23kDa
Observed MW:	28kDa

Anti-FADD Antibody [KO Validated] (CAB18044)

Western blot analysis of extracts from normal (control) and FADD knockout (KO) HeLa cells, using FADD antibody (CAB18044) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Synonyms:

GIG3, MORT1, FADD, FADD

Background:

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

UniProt Protein Function:	FADD: an adaptor molecule that mediates apoptosis. Recruited through its C-terminal death domain by Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TRAIL-receptor, participating in the death signaling initiated by these receptors. Interaction with the receptors unmasks the N-terminal effector domain of this protein, which recruits caspase-8, and thereby activates the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
UniProt Protein Details:	Protein type:Apoptosis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 11q13.3 Cellular Component: neuron projection; CD95 death-inducing signaling complex; cytosol; lipid raft Molecular Function:identical protein binding; protein binding; protease binding; death receptor binding; protein complex binding; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding Biological Process: viral reproduction; positive regulation of apoptosis; apoptosis; positive regulation of T cell mediated cytotoxicity; positive regulation of proteolysis; protein heterooligomerization; T cell differentiation in the thymus; toll-like receptor 3 signaling pathway; positive regulation of activated T cell proliferation; positive regulation of interleukin-8 production; T cell homeostasis; positive regulation of macrophage differentiation; toll-like receptor 4 signaling pathway; defense response to virus; positive regulation of adaptive immune response; spleen development; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; thymus development; MyD88-independent toll-like receptor signaling pathway; positive regulation of tumor necrosis factor production; lymph node development; positive regulation of interferon-gamma production; induction of apoptosis via death domain receptors; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter Disease: Infections, Recurrent, With Encephalopathy, Hepatic Dysfunction, And Cardiovascular Malformations
NCBI Summary:	The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13158
NCBI GenInfo Identifier:	2498355
NCBI Gene ID:	8772
NCBI Accession:	Q13158.1
UniProt Secondary Accession:	Q13158,Q14866, Q6IBR4,
UniProt Related Accession:	Q13158
Molecular Weight:	208
NCBI Full Name:	FAS-associated death domain protein
NCBI Synonym Full Names:	Fas (TNFRSF6)-associated via death domain
NCBI Official Symbol:	FADD
NCBI Official Synonym Symbols:	GIG3; MORT1
NCBI Protein Information:	FAS-associated death domain protein; growth-inhibiting gene 3 protein; mediator of receptor induced toxicity; mediator of receptor-induced toxicity; Fas-associating protein with death domain; Fas-associating death domain-containing protein
UniProt Protein Name:	FAS-associated death domain protein
UniProt Synonym Protein Names:	FAS-associating death domain-containing protein; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity; Protein FADD
Protein Family:	Fas-associated death domain protein
UniProt Gene Name:	FADD
UniProt Entry Name:	FADD_HUMAN

Antibodies	ELISA Kits
Anti-FADD Antibody (CAB16459)	Human FADD (FAS-associated death domain protein) ELISA Kit (HUFI07929)
Anti-FADD Antibody (CAB5819)	FADD Colorimetric Cell-Based ELISA Kit
Anti-FADD Antibody (CAB18082)[KO Validated]	FADD (Phospho-Ser194) Colorimetric Cell-Based ELISA Kit
Anti-FADD Antibody (CAB19049)
Anti-Phospho-FADD-S194 Antibody (CABP1054)