Cell Biology Antibodies 5
Anti-F8A1 Antibody (CAB14806)
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- SKU:
- CAB14806
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-F8A1 Antibody |
| Antibody SKU: | CAB14806 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human F8A1 (NP_036283.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, A-549, Mouse spleen, Rat lung, Rat spleen |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human F8A1 (NP_036283.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AALR DLGQ PAAA AGHF QRAA QLQL PQLP LAAL QALG EAAS CQLL ARDY TGAL AVFT RMQR LARE HGSH PVQS LPPP PPPA PQPG PGAT PALP AALL PPNS G |
| Gene ID: | 8263 |
| Uniprot: | P23610 |
| Cellular Location: | |
| Calculated MW: | 39kDa |
| Observed MW: | 39kDa |
| Synonyms: | F8A1, DXS522E, F8A, HAP40 |
| Background: | This gene is contained entirely within intron 22 of the factor VIII gene; spans less than 2 kb, and is transcribed in the direction opposite of factor VIII. A portion of intron 22 (int22h), containing F8A, is repeated twice extragenically closer to the Xq telomere. Although its function is unknown, the observation that this gene is conserved in the mouse implies it has some function. Unlike factor VIII, this gene is transcribed abundantly in a wide variety of cell types. |
| UniProt Protein Function: | F8A1: Not known. Possible housekeeping role. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: Xq28 Cellular Component: nucleus |
| NCBI Summary: | This gene is part of a region that is repeated three times on chromosome X, once in intron 22 of the F8 gene and twice closer to the Xq telomere. This record represents the most telomeric copy. Although its function is unknown, the observation that this gene is conserved in the mouse implies it has some function. Unlike factor VIII, this gene is transcribed abundantly in a wide variety of cell types. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P23610 |
| NCBI GenInfo Identifier: | 55977780 |
| NCBI Gene ID: | 474384 |
| NCBI Accession: | P23610.2 |
| UniProt Secondary Accession: | P23610,Q5HY66, Q8IXP3, |
| UniProt Related Accession: | P23610 |
| Molecular Weight: | 39,103 Da |
| NCBI Full Name: | Factor VIII intron 22 protein |
| NCBI Synonym Full Names: | coagulation factor VIII-associated 3 |
| NCBI Official Symbol: | F8A3 |
| NCBI Protein Information: | factor VIII intron 22 protein |
| UniProt Protein Name: | Factor VIII intron 22 protein |
| UniProt Synonym Protein Names: | CpG island protein |
| Protein Family: | Factor VIII intron 22 protein |
| UniProt Gene Name: | F8A1 |
| UniProt Entry Name: | F8I2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using F8A1 antibody (CAB14806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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