Cardiovascular Antibodies
Anti-F2RL3 Antibody (CAB8471)
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- SKU:
- CAB8471
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cardiovascular
Description
| Antibody Name: | Anti-F2RL3 Antibody |
| Antibody SKU: | CAB8471 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-150 of human F2RL3 (NP_003941.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, HT-29, Raji, B cells, Mouse spleen, Mouse lung, Mouse skeletal muscle, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-150 of human F2RL3 (NP_003941.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TQTP SVYD ESGS TGGG DDST PSIL PAPR GYPG QVCA NDSD TLEL PDSS RALL LGWV PTRL VPAL YGLV LVVG LPAN GLAL WVLA TQAP RLPS TMLL MNLA AADL LLAL ALPP RIAY HLRG QRWP FGEA ACR |
| Gene ID: | 9002 |
| Uniprot: | Q96RI0 |
| Cellular Location: | Cell membrane, Multi-pass membrane protein |
| Calculated MW: | 41kDa |
| Observed MW: | 41kDa |
| Synonyms: | F2RL3, PAR4 |
| Background: | Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL3 is also a member of the protease-activated receptor family. F2RL3 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL3 is activated by thrombin and trypsin. |
| UniProt Protein Function: | PAR4: a G-protein coupled receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Plays a role in platelets activation. Thrombin requires PAR4 for full spreading on a fibrinogen matrix. Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets. A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: 19p12 Cellular Component: extracellular region; integral to plasma membrane; plasma membrane Molecular Function:thrombin receptor activity Biological Process: blood coagulation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); platelet activation; platelet dense granule organization and biogenesis; positive regulation of release of sequestered calcium ion into cytosol; response to wounding; signal transduction |
| NCBI Summary: | Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL3 is also a member of the protease-activated receptor family. F2RL3 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL3 is activated by thrombin and trypsin. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q96RI0 |
| NCBI GenInfo Identifier: | 116242700 |
| NCBI Gene ID: | 9002 |
| NCBI Accession: | Q96RI0.3 |
| UniProt Secondary Accession: | Q96RI0,O76067, Q6DK42, |
| UniProt Related Accession: | Q96RI0 |
| Molecular Weight: | 41,133 Da |
| NCBI Full Name: | Proteinase-activated receptor 4 |
| NCBI Synonym Full Names: | F2R like thrombin/trypsin receptor 3 |
| NCBI Official Symbol: | F2RL3 |
| NCBI Official Synonym Symbols: | PAR4 |
| NCBI Protein Information: | proteinase-activated receptor 4 |
| UniProt Protein Name: | Proteinase-activated receptor 4 |
| UniProt Synonym Protein Names: | Coagulation factor II receptor-like 3; Thrombin receptor-like 3 |
| Protein Family: | Proteinase-activated receptor |
| UniProt Gene Name: | F2RL3 |
| UniProt Entry Name: | PAR4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using F2RL3 antibody (CAB8471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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