Name: Anti-F13A1 Antibody (CAB1461)
Brand: Cardiovascular Antibodies
SKU: CAB1461
Availability: OutOfStock

Antibody Name:	Anti-F13A1 Antibody
Antibody SKU:	CAB1461
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 600-732 of human F13A1 (NP_000120.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, SH-SY5Y, BxPC-3, Mouse testis, Rat brain, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 600-732 of human F13A1 (NP_000120.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LEQA SLHF FVTA RINE TRDV LAKQ KSTV LTIP EIII KVRG TQVV GSDM TVTV EFTN PLKE TLRN VWVH LDGP GVTR PMKK MFRE IRPN STVQ WEEV CRPW VSGH RKLI ASMS SDSL RHVY GELD VQIQ RRPS M
Gene ID:	2162
Uniprot:	P00488
Cellular Location:	Cytoplasm, Secreted
Calculated MW:	83kDa
Observed MW:	83kDa

Synonyms:

F13A1, F13A

Background:

This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion.

UniProt Protein Function:	F13A1: Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl- epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD). FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. Belongs to the transglutaminase superfamily. Transglutaminase family.
UniProt Protein Details:	Protein type:Transferase; EC 2.3.2.13 Chromosomal Location of Human Ortholog: 6p25.3-p24.3 Cellular Component: extracellular region Molecular Function:protein-glutamine gamma-glutamyltransferase activity; metal ion binding Biological Process: platelet activation; platelet degranulation; peptide cross-linking; blood coagulation Disease: Thrombophilia Due To Thrombin Defect; Myocardial Infarction, Susceptibility To; Factor Xiii, A Subunit, Deficiency Of
NCBI Summary:	This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]
UniProt Code:	P00488
NCBI GenInfo Identifier:	119395709
NCBI Gene ID:	2162
NCBI Accession:	NP_000120.2
UniProt Secondary Accession:	P00488,Q59HA7, Q8N6X2, Q96P24, Q9BX29,
UniProt Related Accession:	P00488
Molecular Weight:	312,000
NCBI Full Name:	coagulation factor XIII A chain
NCBI Synonym Full Names:	coagulation factor XIII, A1 polypeptide
NCBI Official Symbol:	F13A1
NCBI Official Synonym Symbols:	F13A
NCBI Protein Information:	coagulation factor XIII A chain; TGase; factor XIIIa; fibrinoligase; FSF, A subunit; coagulation factor XIIIa; transglutaminase A chain; transglutaminase. plasma; fibrin stabilizing factor, A subunit; coagulation factor XIII, A polypeptide; protein-glutamine gamma-glutamyltransferase A chain; bA525O21.1 (coagulation factor XIII, A1 polypeptide)
UniProt Protein Name:	Coagulation factor XIII A chain
UniProt Synonym Protein Names:	Protein-glutamine gamma-glutamyltransferase A chain; Transglutaminase A chain
Protein Family:	36 kDa major membrane protein
UniProt Gene Name:	F13A1
UniProt Entry Name:	F13A_HUMAN

Western blot analysis of extracts of various cell lines, using F13A1 antibody (CAB1461) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies	ELISA Kits
Anti-F13A1 Antibody (CAB16847)	Human F13A / Factor XIII ELISA Kit
Cleaved-F13A1 (G39) Antibody (PACO03649)	Human F13A1 (Coagulation Factor 13 A1 Polypeptide) CLIA Kit (HUES00481)
	Human F13A1 (Coagulation Factor 13 A1 Polypeptide) ELISA Kit (HUES01908)
	Human Coagulation factor XIII A chain (F13A1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)