Cardiovascular Antibodies
Anti-F12 Antibody (CAB1691)
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- SKU:
- CAB1691
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cardiovascular
Description
| Antibody Name: | Anti-F12 Antibody |
| Antibody SKU: | CAB1691 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 336-615 of human F12 (NP_000496.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HepG2, MCF7, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 336-615 of human F12 (NP_000496.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QTPG ALPA KREQ PPSL TRNG PLSC GQRL RKSL SSMT RVVG GLVA LRGA HPYI AALY WGHS FCAG SLIA PCWV LTAA HCLQ DRPA PEDL TVVL GQER RNHS CEPC QTLA VRSY RLHE AFSP VSYQ HDLA LLRL QEDA DGSC ALLS PYVQ PVCL PSGA ARPS ETTL CQVA GWGH QFEG AEEY ASFL QEAQ VPFL SLER CSAP DVHG SSIL PGML CAGF LEGG TDAC QGDS GGPL VCED QAAE RRLT LQGI ISWG SGCG DRNK PGVY TDVA YYLA WIRE HTVS |
| Gene ID: | 2161 |
| Uniprot: | P00748 |
| Cellular Location: | Secreted |
| Calculated MW: | 67kDa |
| Observed MW: | 71kDa |
| Synonyms: | F12, HAE3, HAEX, HAF |
| Background: | This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. |
| UniProt Protein Function: | F12: Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta- factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. Defects in F12 are the cause of factor XII deficiency (FA12D); also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection). Defects in F12 are the cause of hereditary angioedema type 3 (HAE3); also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g. during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal. Belongs to the peptidase S1 family. |
| UniProt Protein Details: | Protein type:Protease; Secreted; Chaperone; EC 3.4.21.38; Secreted, signal peptide Chromosomal Location of Human Ortholog: 5q35.3 Cellular Component: extracellular region; extracellular space; plasma membrane Molecular Function:misfolded protein binding; protein binding; serine-type endopeptidase activity Biological Process: blood coagulation; blood coagulation, intrinsic pathway; Factor XII activation; fibrinolysis; innate immune response; plasma kallikrein-kinin cascade; positive regulation of blood coagulation; positive regulation of fibrinolysis; protein autoprocessing; protein processing; response to misfolded protein; zymogen activation Disease: Angioedema, Hereditary, Type Iii; Factor Xii Deficiency |
| NCBI Summary: | This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P00748 |
| NCBI GenInfo Identifier: | 317373446 |
| NCBI Gene ID: | 2161 |
| NCBI Accession: | P00748.3 |
| UniProt Secondary Accession: | P00748,P78339, |
| UniProt Related Accession: | P00748 |
| Molecular Weight: | 67,792 Da |
| NCBI Full Name: | Coagulation factor XII |
| NCBI Synonym Full Names: | coagulation factor XII |
| NCBI Official Symbol: | F12 |
| NCBI Official Synonym Symbols: | HAF; HAE3; HAEX |
| NCBI Protein Information: | coagulation factor XII |
| UniProt Protein Name: | Coagulation factor XII |
| UniProt Synonym Protein Names: | Hageman factor; HAF |
| Protein Family: | Coagulation factor |
| UniProt Gene Name: | F12 |
| UniProt Entry Name: | FA12_HUMAN |
 | Western blot analysis of extracts of various cell lines, using F12 antibody (CAB1691) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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