Cell Biology Antibodies 1
Anti-EZR Antibody (CAB0703)
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- SKU:
- CAB0703
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-EZR Antibody |
| Antibody SKU: | CAB0703 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human EZR (NP_003370.2). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | M21, SW480, U-251MG, 293T, Mouse intestine, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-470 of human EZR (NP_003370.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RLQD YEEK TKKA EREL SEQI QRAL QLEE ERKR AQEE AERL EADR MAAL RAKE ELER QAVD QIKS QEQL AAEL AEYT AKIA LLEE ARRR KEDE VEEW QHRA KEAQ DDLV KTKE ELHL VMTA P |
| Gene ID: | 7430 |
| Uniprot: | P15311 |
| Cellular Location: | Apical cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cell cortex, cytoskeleton, microvillus membrane, ruffle membrane |
| Calculated MW: | 69kDa |
| Observed MW: | 90kDa |
| Synonyms: | CVIL, CVL, HEL-S-105, VIL2, EZR, Ezrin, ezrin |
| Background: | The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. |
| UniProt Protein Function: | Ezrin: a member of the ERM family which includes radixin and moesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Plays a key role in cell surface structure adhesion, migration, and organization. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 6q25.3 Cellular Component: cortical cytoskeleton; extracellular space; microvillus; focal adhesion; basolateral plasma membrane; T-tubule; actin filament; cytosol; lipid raft; actin cytoskeleton; ruffle; extrinsic to membrane; membrane; apical part of cell; microvillus membrane; apical plasma membrane; nucleolus; plasma membrane; uropod; vesicle; filopodium Molecular Function:actin filament binding; protein domain specific binding; protein binding; cell adhesion molecule binding Biological Process: actin filament bundle formation; axon guidance; microvillus biogenesis; regulation of cell shape; leukocyte adhesion; filopodium formation; cytoskeletal anchoring; receptor internalization; membrane to membrane docking; establishment of epithelial cell polarity |
| NCBI Summary: | The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P15311 |
| NCBI GenInfo Identifier: | 125987826 |
| NCBI Gene ID: | 7430 |
| NCBI Accession: | P15311.4 |
| UniProt Secondary Accession: | P15311,P23714, Q4VX75, Q96CU8, Q9NSJ4, E1P5A8, |
| UniProt Related Accession: | P15311 |
| Molecular Weight: | 586 |
| NCBI Full Name: | Ezrin |
| NCBI Synonym Full Names: | ezrin |
| NCBI Official Symbol: | EZR |
| NCBI Official Synonym Symbols: | CVL; CVIL; VIL2; HEL-S-105 |
| NCBI Protein Information: | ezrin; p81; villin-2; cytovillin 2; villin 2 (ezrin); epididymis secretory protein Li 105 |
| UniProt Protein Name: | Ezrin |
| UniProt Synonym Protein Names: | Cytovillin; Villin-2; p81 |
| Protein Family: | Ezrin |
| UniProt Gene Name: | EZR |
| UniProt Entry Name: | EZRI_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EZR antibody (CAB0703) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human stomach using EZR antibody (CAB0703) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using EZR antibody (CAB0703) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using EZR antibody (CAB0703) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug EZR antibody (CAB0703). Western blot was performed from the immunoprecipitate using EZR antibody (CAB0703) at a dilition of 1:1000. |
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