Cell Biology Antibodies 17

Anti-EZH2 Antibody (CAB5743)

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SKU:
CAB5743
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-EZH2 Antibody
Antibody SKU:CAB5743
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EZH2 (NP_001190176.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, SW620
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EZH2 (NP_001190176.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGQT GKKS EKGP VCWR KRVK SEYM RLRQ LKRF RRAD EVKS MFSS NRQK ILER TEIL NQEW KQRR IQPV HILT SVSS LRGT RECS VTSD LDFP TQVI PLKT LNAV ASVP IMYS WSPL QQNF MVED ETVL HNIP YMGD EVLD QDGT FIEE LIKN YDGK VHGD RECG FIND EIFV ELVN ALGQ YNDD DDDD DGDD PEER EEKQ KDLE DHRD DKES RPPR KFPS DKIF EAIS SMFP DKGT AEEL KEKY KELT EQ
Gene ID:2146
Uniprot:Q15910
Cellular Location:Nucleus
Calculated MW:79kDa/81kDa/84kDa/85kDa/86kDa
Observed MW:105kDa
Synonyms:ENX-1, ENX1, EZH1, EZH2b, KMT6, KMT6A, WVS, WVS2, EZH2, KMT6 / EZH2
Background:This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
UniProt Protein Function:EZH2: a histone H3 Lys 27 (H3K27) methyltransferase and Polycomb group protein with oncogenic activity. A master regulatory protein that plays a critical role in development. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates K9 and K27 of histone H3, leading to transcriptional repression of the affected target genes. PRC2 includes the Ezh2, EED, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The recruitment of PRC2 to a promoter leads to increased levels of di- and trimethylation of H3K27 (H3K27me2/3), regulating the balance between self-renewal and differentiation of embryonic stem cells (ESCs). The recruitment of PRC2 to promoters in ES cells is interdependent on Jarid 2. ESCs lacking the PRC2 component EED are deficient in Jarid2 promoter activity, while the knockdown of Jarid 2 reduces PRC2 at its target promoters. Able to mono-, di- and trimethylate K27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, p14ARF and retinoic acid target genes. Binds ATRX via the SET domain. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. Interacts with PRAME. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. Expression decreases during senescence of embryonic fibroblasts. Expression peaks at the G1/S phase boundary. Expression is induced by E2F1, E2F2 and E2F3. Expression is reduced in cells subject to numerous types of stress including UV-, IR- and bleomycin-induced DNA damage and by activation of p53. It has been reported that phosphorylation by AKT1 on S21 reduces methyltransferase activity.
UniProt Protein Details:

Protein type:EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: ESC/E(Z) complex; nuclear chromatin; nucleoplasm; nucleus

Molecular Function:chromatin binding; chromatin DNA binding; DNA binding; histone lysine N-methyltransferase activity (H3-K27 specific); histone methyltransferase activity; histone-lysine N-methyltransferase activity; protein binding; protein-lysine N-methyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; histone methylation; negative regulation of gene expression, epigenetic; negative regulation of retinoic acid receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of GTPase activity; positive regulation of MAP kinase activity; regulation of circadian rhythm; regulation of transcription, DNA-dependent

Disease: Weaver Syndrome

NCBI Summary:This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]
UniProt Code:Q15910
NCBI GenInfo Identifier:3334180
NCBI Gene ID:2146
NCBI Accession:Q15910.2
UniProt Secondary Accession:Q15910,Q15755, Q75MG3, Q92857, Q96FI6, B2RAQ1, B3KS30 B7Z1D6, B7Z7L6,
UniProt Related Accession:Q15910
Molecular Weight:79,621 Da
NCBI Full Name:Histone-lysine N-methyltransferase EZH2
NCBI Synonym Full Names:enhancer of zeste 2 polycomb repressive complex 2 subunit
NCBI Official Symbol:EZH2  
NCBI Official Synonym Symbols:WVS; ENX1; KMT6; WVS2; ENX-1; EZH2b; KMT6A  
NCBI Protein Information:histone-lysine N-methyltransferase EZH2
UniProt Protein Name:Histone-lysine N-methyltransferase EZH2
UniProt Synonym Protein Names:ENX-1; Enhancer of zeste homolog 2
Protein Family:Histone-lysine N-methyltransferase
UniProt Gene Name:EZH2  
Anti-EZH2 Antibody (CAB5743)Western blot - EZH2 Polyclonal Antibody (CAB5743)
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