Epigenetics & Nuclear Signaling Antibodies 2

Anti-EYA3 Antibody (CAB15056)

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SKU:
CAB15056
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-EYA3 Antibody
Antibody SKU:CAB15056
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EYA3 (NP_001981.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Rat
Positive Samples:HT-29, Rat eye
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EYA3 (NP_001981.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEEE QDLP EQPV KKAK MQES GEQT ISQV SNPD VSDQ KPET SSLA SNLP MSEE IMTC TDYI PRSS NDYT SQMY SAKP YAHI LSVP VSET AYPG QTQY QTLQ QTQP YAVY PQAT QTYG LPPF GALW PGMK PESG LIQT PSPS QHSV LTCT TGLT TSQP SPAH YSYP IQAS STNA SLIS TSST IANI PAAA VASI SNQD YPTY TILG QNQY QACY PSSS FGVT GQTN SDAE STTL AATT YQSE KPSV MAPA PAAQ RLSS GDPS TSPS LSQT TPSK DTDD QSRK NMTS KNRG KRKA DATS SQDS
Gene ID:2140
Uniprot:Q99504
Cellular Location:Cytoplasm, Nucleus
Calculated MW:48kDa/56kDa/57kDa/58kDa/62kDa
Observed MW:57kDa/58kDa
Synonyms:EYA3
Background:This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants.
UniProt Protein Function:Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1 (PubMed:19234442, PubMed:19351884). Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity. May be involved in development of the eye.
NCBI Summary:This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013]
UniProt Code:Q99504
NCBI GenInfo Identifier:543173153
NCBI Gene ID:2140
NCBI Accession:NP_001269489.1
UniProt Secondary Accession:Q99504,O95463, Q8IVX7, Q99813, A8K190, B4DIR7, B4DNZ7
UniProt Related Accession:Q99504
Molecular Weight:57,827 Da
NCBI Full Name:eyes absent homolog 3 isoform b
NCBI Synonym Full Names:EYA transcriptional coactivator and phosphatase 3
NCBI Official Symbol:EYA3  
NCBI Protein Information:eyes absent homolog 3
UniProt Protein Name:Eyes absent homolog 3
Protein Family:Eyes absent
UniProt Gene Name:EYA3  
Anti-EYA3 Antibody (CAB15056)Western blot analysis of extracts of various cell lines, using EYA3 antibody (CAB15056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-EYA3 Antibody (CAB15056)Immunofluorescence analysis of U2OS cells using EYA3 antibody (CAB15056) at dilution of 1:100. Blue: DAPI for nuclear staining.
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