Name: Anti-EYA2 Antibody (CAB15055)
Brand: Epigenetics & Nuclear Signaling Antibodies 2
SKU: CAB15055
Availability: OutOfStock

Antibody Name:	Anti-EYA2 Antibody
Antibody SKU:	CAB15055
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300-400 of human EYA2 (NP_005235.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	293T, A-549

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300-400 of human EYA2 (NP_005235.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TSVR IGLM MEEM IFNL ADTH LFFN DLED CDQI HVDD VSSD DNGQ DLST YNFS ADGF HSSA PGAN LCLG SGVH GGVD WMRK LAFR YRRV KEMY NTYK NNVG G
Gene ID:	2139
Uniprot:	O00167
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	50kDa/56kDa/59kDa
Observed MW:	59kDa

Synonyms:	EYA2, EAB1
Background:	This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may be post-translationally modified and may play a role in eye development. A similar protein in mice can act as a transcriptional activator. Alternative splicing results in multiple transcript variants, but the full-length natures of all of these variants have not yet been determined.

UniProt Protein Function:	EYA2: Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. Together with SIX1 and DACH2 seem to be involved in myogenesis. May be involved in development of the eye. Interaction with GNAZ and GNAI2 prevents nuclear translocation and transcriptional activity. Belongs to the HAD-like hydrolase superfamily. EYA family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48; Apoptosis; Cell development/differentiation Chromosomal Location of Human Ortholog: 20q13.1 Cellular Component: nucleoplasm Molecular Function:magnesium ion binding; protein binding; protein tyrosine phosphatase activity Biological Process: histone dephosphorylation; mesodermal cell fate specification
NCBI Summary:	This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may be post-translationally modified and may play a role in eye development. A similar protein in mice can act as a transcriptional activator. Alternative splicing results in multiple transcript variants, but the full-length natures of all of these variants have not yet been determined. [provided by RefSeq, Jul 2009]
UniProt Code:	O00167
NCBI GenInfo Identifier:	20455483
NCBI Gene ID:	2139
NCBI Accession:	O00167.2
UniProt Secondary Accession:	O00167,Q5JSW8, Q86U84, Q96CV6, Q96H97, Q99503, Q99812 Q9BWF6, Q9H4S3, Q9H4S9, Q9NPZ4, Q9UIX7,
UniProt Related Accession:	O00167
Molecular Weight:	50,533 Da
NCBI Full Name:	Eyes absent homolog 2
NCBI Synonym Full Names:	EYA transcriptional coactivator and phosphatase 2
NCBI Official Symbol:	EYA2
NCBI Official Synonym Symbols:	EAB1
NCBI Protein Information:	eyes absent homolog 2
UniProt Protein Name:	Eyes absent homolog 2
Protein Family:	Eyes absent
UniProt Gene Name:	EYA2
UniProt Entry Name:	EYA2_HUMAN

Western blot analysis of extracts of various cell lines, using EYA2 antibody (CAB15055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

EYA2 Antibody (PACO09177)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)