Name: Anti-EXTL3 Antibody (CAB3857)
Brand: Cell Biology Antibodies 9
SKU: CAB3857
Availability: OutOfStock

Antibody Name:	Anti-EXTL3 Antibody
Antibody SKU:	CAB3857
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 52-220 of human EXTL3 (NP_001431.1).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Mouse pancreas, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 52-220 of human EXTL3 (NP_001431.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TTLD EADE AGKR IFGP RVGN ELCE VKHV LDLC RIRE SVSE ELLQ LEAK RQEL NSEI AKLN LKIE ACKK SIEN AKQD LLQL KNVI SQTE HSYK ELMA QNQP KLSL PIRL LPEK DDAG LPPP KATR GCRL HNCF DYSR CPLT SGFP VYVY DSDQ FVFG SYLD PLVK QAFQ A
Gene ID:	2137
Uniprot:	O43909
Cellular Location:	Endoplasmic reticulum membrane, Single-pass type II membrane protein
Calculated MW:	104kDa
Observed MW:	105kDa

Synonyms:	EXTL3, BOTV, EXTL1L, EXTR1, REGR, RPR, ISDNA
Background:	This gene encodes a single-pass membrane protein which functions as a glycosyltransferase. The encoded protein catalyzes the transfer of N-acetylglucosamine to glycosaminoglycan chains. This reaction is important in heparin and heparan sulfate synthesis. Alternative splicing results in the multiple transcript variants.

UniProt Protein Function:	EXTL3: Probable glycosyltransferase. Belongs to the glycosyltransferase 47 family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Transferase; EC 2.4.1.223; Glycan Metabolism - heparan sulfate biosynthesis Chromosomal Location of Human Ortholog: 8p21 Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane Molecular Function:metal ion binding; glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Biological Process: glycosaminoglycan biosynthetic process; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; unfolded protein response; heparan sulfate proteoglycan biosynthetic process; positive regulation of cell growth
NCBI Summary:	This gene encodes a single-pass membrane protein which functions as a glycosyltransferase. The encoded protein catalyzes the transfer of N-acetylglucosamine to glycosaminoglycan chains. This reaction is important in heparin and heparan sulfate synthesis. Alternative splicing results in the multiple transcript variants. [provided by RefSeq, Nov 2012]
UniProt Code:	O43909
NCBI GenInfo Identifier:	6166159
NCBI Gene ID:	2137
NCBI Accession:	O43909.1
UniProt Secondary Accession:	O43909,O00225, Q53XT3, D3DST8,
UniProt Related Accession:	O43909
Molecular Weight:	Observed MW 105kDaCalculated MW 104kDa
NCBI Full Name:	Exostosin-like 3
NCBI Synonym Full Names:	exostosin-like glycosyltransferase 3
NCBI Official Symbol:	EXTL3
NCBI Official Synonym Symbols:	RPR; BOTV; REGR; EXTR1; EXTL1L
NCBI Protein Information:	exostosin-like 3; reg receptor; EXT-related 1; exostosin tumor-like 3; exostoses (multiple)-like 3; putative tumor suppressor protein EXTL3; hereditary multiple exostoses gene isolog; glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
UniProt Protein Name:	Exostosin-like 3
UniProt Synonym Protein Names:	EXT-related protein 1; Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase; Hereditary multiple exostoses gene isolog; Multiple exostosis-like protein 3; Putative tumor suppressor protein EXTL3
Protein Family:	Exostosin
UniProt Gene Name:	EXTL3
UniProt Entry Name:	EXTL3_HUMAN

	Western blot analysis of extracts of various cell lines, using EXTL3 antibody (CAB3857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat kidney using EXTL3 Rabbit pAb (CAB3857) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using EXTL3 Rabbit pAb (CAB3857) at dilution of 1:100 (40x lens).