Epigenetics & Nuclear Signaling Antibodies 1

Anti-EXOSC3 Antibody (CAB11623)

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SKU:
CAB11623
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-EXOSC3 Antibody
Antibody SKU:CAB11623
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human EXOSC3 (NP_057126.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:A-431, HeLa, U-87MG, Raji, SGC-7901, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human EXOSC3 (NP_057126.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAEP ASVA AESL AGSR ARAA RTVL GQVV LPGE ELLL PEQE DAEG PGGA VERP LSLN ARAC SRVR VVCG PGLR RCGD RLLV TKCG RLRH KEPG SGSG GGVY WVDS QQKR YVPV KGDH VIGI VTAK SGDI FKVD VGGS EPAS LSYL SFEG ATKR NRPN VQVG DLIY GQFV VANK DMEP EMVC IDSC GRAN GMGV IGQD GLLF KVTL GLIR KLLA PDCE IIQE VGKL YPLE IVFG MNGR IWVK AKTI QQTL ILAN ILEA CEHM TSDQ RKQI FSRL AES
Gene ID:51010
Uniprot:Q9NQT5
Cellular Location:Cytoplasm, Nucleus, nucleolus
Calculated MW:17kDa/29kDa
Observed MW:30kDa
Synonyms:EXOSC3, CGI-102, PCH1B, RRP40, Rrp40p, bA3J10.7, hRrp-40, p10
Background:This gene encodes a non-catalytic component of the human exosome, a complex with 3'-5' exoribonuclease activity that plays a role in numerous RNA processing and degradation activities. Related pseudogenes of this gene are found on chromosome 19 and 21. Alternatively spliced transcript variants encoding different isoforms have been described.
UniProt Protein Function:EXOSC3: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5. Defects in EXOSC3 are the cause of pontocerebellar hypoplasia type 1B (PCH1B). A severe autosomal recessive neurologic disorder characterized by a combination of cerebellar and spinal motor neuron degeneration beginning at birth. There is diffuse muscle weakness, progressive microcephaly, global developmental delay, and brainstem involvement. Belongs to the RRP40 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.1.13.-; Nucleolus; RNA processing; Ribonuclease

Chromosomal Location of Human Ortholog: 9p13.2

Cellular Component: cytoplasm; cytosol; exosome (RNase complex); nuclear exosome (RNase complex); nucleolus; nucleoplasm; nucleus

Molecular Function:exoribonuclease activity; protein binding; RNA binding

Biological Process: DNA deamination; exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); isotype switching; regulation of mRNA stability; rRNA processing

NCBI Summary:This gene encodes a non-catalytic component of the human exosome, a complex with 3'-5' exoribonuclease activity that plays a role in numerous RNA processing and degradation activities. Related pseudogenes of this gene are found on chromosome 19 and 21. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2012]
UniProt Code:Q9NQT5
NCBI GenInfo Identifier:14285758
NCBI Gene ID:51010
NCBI Accession:Q9NQT5.3
UniProt Secondary Accession:Q9NQT5,Q5QP85, Q9Y3A8, A8K0K6,
UniProt Related Accession:Q9NQT5
Molecular Weight:30kDa
NCBI Full Name:Exosome complex component RRP40
NCBI Synonym Full Names:exosome component 3
NCBI Official Symbol:EXOSC3  
NCBI Official Synonym Symbols:p10; PCH1B; RRP40; Rrp40p; CGI-102; hRrp-40; bA3J10.7  
NCBI Protein Information:exosome complex component RRP40
UniProt Protein Name:Exosome complex component RRP40
UniProt Synonym Protein Names:Exosome component 3; Ribosomal RNA-processing protein 40; p10
Protein Family:Exosome complex component
UniProt Gene Name:EXOSC3  
Anti-EXOSC3 Antibody (CAB11623)Western blot analysis of extracts of various cell lines, using EXOSC3 antibody (CAB11623) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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