Epigenetics & Nuclear Signaling Antibodies 1

Anti-EXOSC2 Antibody (CAB10450)

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SKU:
CAB10450
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-EXOSC2 Antibody
Antibody SKU:CAB10450
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human EXOSC2 (NP_055100.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, HeLa, Mouse liver, Mouse spleen, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human EXOSC2 (NP_055100.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAME MRLP VARK PLSE RLGR DTKK HLVV PGDT ITTD TGFM RGHG TYMG EEKL IASV AGSV ERVN KLIC VKAL KTRY IGEV GDIV VGRI TEVQ QKRW KVET NSRL DSVL LLSS MNLP GGEL RRRS AEDE LAMR GFLQ EGDL ISAE VQAV FSDG AVSL HTRS LKYG KLGQ GVLV QVSP SLVK RQKT HFHD LPCG ASVI LGNN GFIW IYPT PEHK EEEA GGFI ANLE PVSL ADRE VISR LRNC IISL VTQR MMLY DTSI LYCY EASL PHQI KDIL KPEI MEEI VMET RQRL LEQE G
Gene ID:23404
Uniprot:Q13868
Cellular Location:Cytoplasm, Nucleus, nucleolus
Calculated MW:29kDa/30kDa/32kDa
Observed MW:33kDa
Synonyms:EXOSC2, RRP4, Rrp4p, hRrp4p, p7
Background:
UniProt Protein Function:RRP4: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC2 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC4 and EXOSC7.
UniProt Protein Details:

Protein type:RNA processing; EC 3.1.13.-; Ribonuclease; Nucleolus

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: cytoplasm; nucleolus; exosome (RNase complex); cytosol; nucleus; nuclear exosome (RNase complex)

Molecular Function:protein binding; 3'-5'-exoribonuclease activity; exoribonuclease activity; 7S RNA binding

Biological Process: gene expression; exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); positive regulation of cell growth; rRNA processing; mRNA catabolic process, deadenylation-dependent decay

UniProt Code:Q13868
NCBI GenInfo Identifier:13878748
NCBI Gene ID:23404
NCBI Accession:Q13868.2
UniProt Secondary Accession:Q13868,Q9NUY4, A3KFL3, B4DKK6,
UniProt Related Accession:Q13868
Molecular Weight:293
NCBI Full Name:Exosome complex component RRP4
NCBI Synonym Full Names:exosome component 2
NCBI Official Symbol:EXOSC2  
NCBI Official Synonym Symbols:p7; RRP4; Rrp4p; hRrp4p  
NCBI Protein Information:exosome complex component RRP4; exosome complex exonuclease RRP4; ribosomal RNA-processing protein 4; homolog of yeast RRP4 (ribosomal RNA processing 4), 3'-5'-exoribonuclease; homolog of yeast RRP4 (ribosomal RNA processing 4), 3' 5' exoribonuclease (RRP4)
UniProt Protein Name:Exosome complex component RRP4
UniProt Synonym Protein Names:Exosome component 2; Ribosomal RNA-processing protein 4
UniProt Gene Name:EXOSC2  
UniProt Entry Name:EXOS2_HUMAN
Anti-EXOSC2 Antibody (CAB10450)Western blot analysis of extracts of various cell lines, using EXOSC2 antibody (CAB10450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-EXOSC2 Antibody (CAB10450)Immunofluorescence analysis of U2OS cells using EXOSC2 Rabbit pAb (CAB10450) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-EXOSC2 Antibody (CAB10450)Immunofluorescence analysis of L929 cells using EXOSC2 Rabbit pAb (CAB10450) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-EXOSC2 Antibody (CAB10450)Immunofluorescence analysis of H9C2 cells using EXOSC2 Rabbit pAb (CAB10450) at dilution of 1:100. Blue: DAPI for nuclear staining.
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