Anti-EXOC3 Antibody (CAB20925)
- SKU:
- CAB20925
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-EXOC3 Antibody (CAB20925) |
| Antibody SKU: | CAB20925 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunoprecipitation |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of Human EXOC3. |
| Application: | Western blotting, Immunoprecipitation |
| Recommended Dilution: | WB 1:500 - 1:2000 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, MCF7, U-251MG, Mouse brain, Rat brain |
| Immunogen: | Recombinant protein of Human EXOC3. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | |
| Calculated MW: | 73kDa/85kDa/86kDa |
| Observed MW: | 85kDa |
| Synonyms: | EXOC3, SEC6, SEC6L1, Sec6p, EXOC3 |
| Background: | The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. |
 | Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug EXOC3 antibody . Western blot was performed from the immunoprecipitate using EXOC3 antibody at a dilition of 1:1000. |
 | Western blot analysis of extracts of various cell lines, using EXOC3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
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