Name: Anti-EXO1 Antibody (CAB1941)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB1941
Availability: OutOfStock

Antibody Name:	Anti-EXO1 Antibody
Antibody SKU:	CAB1941
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 601-846 of human EXO1 (NP_006018.4).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	HeLa, Jurkat, THP-1, K-562

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 601-846 of human EXO1 (NP_006018.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TLGT LRSC FSWS GGLG DFSR TPSP SPST ALQQ FRRK SDSP TSLP ENNM SDVS QLKS EESS DDES HPLR EEAC SSQS QESG EFSL QSSN ASKL SQCS SKDS DSEE SDCN IKLL DSQS DQTS KLRL SHFS KKDT PLRN KVPG LYKS SSAD SLST TKIK PLGP ARAS GLSK KPAS IQKR KHHN AENK PGLQ IKLN ELWK NFGF KKDS EKLP PCKK PLSP VRDN IQLT PEAE EDIF NKPE CGRV QRAI FQ
Gene ID:	9156
Uniprot:	Q9UQ84
Cellular Location:	Nucleus
Calculated MW:	89kDa/94kDa
Observed MW:	115kDa

Synonyms:	EXO1, HEX1, hExoI
Background:	This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms.

UniProt Protein Function:	EXO1: 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch- containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.1.-.-; Hydrolase Chromosomal Location of Human Ortholog: 1q43 Cellular Component: nucleoplasm; nucleus Molecular Function:5'-3' exonuclease activity; protein binding; double-stranded DNA specific 5'-3' exodeoxyribonuclease activity; structure-specific DNA binding; DNA binding; 5'-3' exodeoxyribonuclease activity; single-stranded DNA specific 5'-3' exodeoxyribonuclease activity; metal ion binding; exonuclease activity; ribonuclease H activity; flap endonuclease activity Biological Process: humoral immune response mediated by circulating immunoglobulin; mismatch repair; meiotic cell cycle; somatic hypermutation of immunoglobulin genes; isotype switching; DNA repair; DNA catabolic process, exonucleolytic; DNA catabolic process, endonucleolytic; DNA recombination
NCBI Summary:	This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UQ84
NCBI GenInfo Identifier:	85700954
NCBI Gene ID:	9156
NCBI Accession:	Q9UQ84.2
UniProt Secondary Accession:	Q9UQ84,O60545, O75214, O75466, Q5T396, Q96IJ1, Q9UG38 Q9UNW0,
UniProt Related Accession:	Q9UQ84
Molecular Weight:	846
NCBI Full Name:	Exonuclease 1
NCBI Synonym Full Names:	exonuclease 1
NCBI Official Symbol:	EXO1
NCBI Official Synonym Symbols:	HEX1; hExoI
NCBI Protein Information:	exonuclease 1; hExo1; rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1
UniProt Protein Name:	Exonuclease 1
UniProt Synonym Protein Names:	Exonuclease I; hExoI
Protein Family:	Exonuclease
UniProt Gene Name:	EXO1
UniProt Entry Name:	EXO1_HUMAN

	Western blot analysis of extracts of various cell lines, using EXO1 Antibody (CAB1941) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human esophageal cancer using EXO1 antibody (CAB1941) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using EXO1 antibody (CAB1941) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using EXO1 antibody (CAB1941) at dilution of 1:100. Blue: DAPI for nuclear staining.