Antibody Name:	Anti-EWSR1 Antibody
Antibody SKU:	CAB1690
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human EWSR1 (NP_001156757.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human EWSR1 (NP_001156757.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TTTA TVTT TQAS YAAQ SAYG TQPA YPAY GQQP AATA PTRP QDGN KPTE TSQP QSST GGYN QPSL GYGQ SNYS YPQV PGSY PMQP VTAP PSYP PTSY SSTQ PTSY DQSS YSQQ NTYG QPSS YGQQ SSYG QQSS YGQQ PPTS YPPQ TGSY SQAP SQYS QQSS SYGQ QSSF RQDH PSSM GVYG QESG GFSG PGEN RSMS GPDN R
Gene ID:	2130
Uniprot:	Q01844
Cellular Location:	Cell membrane, Cytoplasm, Nucleus
Calculated MW:	37kDa/61kDa/62kDa/68kDa
Observed MW:	75kDa

Synonyms:

EWSR1, EWS, EWS-FLI1, bK984G1.4

Background:

This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.

UniProt Protein Function:	EWS: an oncogenic RNA-binding protein. Might normally function as a repressor. Binds RPB3, SF1, calmodulin and RNA. Interacts with and regulated by Pyk2. Relocates from cytoplasm to ribosomes upon Pyk2 activation. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. 5 alternatively-spliced isoforms have been described.
UniProt Protein Details:	Protein type:Oncoprotein; Transcription, coactivator/corepressor; RNA-binding Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: nucleolus; nucleus Molecular Function:identical protein binding; protein binding; RNA binding Disease: Ewing Sarcoma
NCBI Summary:	This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009]
UniProt Code:	Q01844
NCBI GenInfo Identifier:	544261
NCBI Gene ID:	2130
NCBI Accession:	Q01844.1
UniProt Secondary Accession:	Q01844,Q5THL0, Q92635, Q96FE8, Q96MN4, Q96MX4, Q9BWA2 B0QYK1,
UniProt Related Accession:	Q01844
Molecular Weight:	62,508 Da
NCBI Full Name:	RNA-binding protein EWS
NCBI Synonym Full Names:	EWS RNA binding protein 1
NCBI Official Symbol:	EWSR1
NCBI Official Synonym Symbols:	EWS; EWS-FLI1; bK984G1.4
NCBI Protein Information:	RNA-binding protein EWS
UniProt Protein Name:	RNA-binding protein EWS
UniProt Synonym Protein Names:	EWS oncogene; Ewing sarcoma breakpoint region 1 protein
Protein Family:	RNA-binding protein
UniProt Gene Name:	EWSR1
UniProt Entry Name:	EWS_HUMAN

	Western blot analysis of extracts of HeLa cells, using EWSR1 antibody (CAB1690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of U2OS cells using EWSR1 antibody (CAB1690) at dilution of 1:100. Blue: DAPI for nuclear staining.